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A4T9L2 (BIOD_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:Mflv_3627
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000079276

Regions

Nucleotide binding108 – 1114ATP By similarity
Nucleotide binding197 – 1993ATP By similarity

Sites

Metal binding161Magnesium 2 By similarity
Metal binding491Magnesium 2 By similarity
Metal binding1081Magnesium 2 By similarity
Binding site411Substrate By similarity
Binding site491ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4T9L2 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 0FE1206A73743177

FASTA22623,051
        10         20         30         40         50         60 
MSILVVTGTG TGIGKTVVTA ALACHARLAG LDVAVCKPIQ TGTRDGDDDL AVVARLAGVE 

        70         80         90        100        110        120 
DLHSLAKFPE PLAPLAAAHR AGADLPSRAA LDDLIAAVDR PGRLTLVEGA GGLLVEIGRG 

       130        140        150        160        170        180 
GVTLRDLAED LRAPVLTVVA PGLGTLNHTV LTLEALAHRD VRSEGLVIGS WPAQPGVAEI 

       190        200        210        220 
DNRDALARLG PVRAALPAGA GTLSGTDFER LSVQAFDEAW VRGLVG

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PYR-GCK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000656 Genomic DNA. Translation: ABP46101.1.
RefSeqYP_001134889.1. NC_009338.1.

3D structure databases

ProteinModelPortalA4T9L2.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4T9L2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000026167; EBMYCP00000025829; EBMYCG00000026162.
GeneID4974945.
GenomeReviewsGene locus Mflv_3627 in contig CP000656_GR.
KEGGmgi:Mflv_3627.
PATRIC18035424. VBIMycGil17082_3687.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0132.
GeneTreeEBGT00050000017652.
HOGENOMHBG650065.
OMADLASRCN.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycMGIL350054:MFLV_3627-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_MYCGI
AccessionPrimary (citable) accession number: A4T9L2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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