A4T9K8 (A4T9K8_MYCGI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Mflv_3633

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABP46107.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	246	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	283	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	379	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A4T9K8 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 85D5024D8807F94F	FASTA	577	63,457
	10 20 30 40 50 60 MTDHEFAVWA PLPQRVRLDV DGTAHPMTRD EDGWWRATVD AAPQARYGFL LDDDPAVLPD 70 80 90 100 110 120 PRSARQPDGV HERSQLWSPP SDGWTDAGWQ GRSIEGAVIY ELHIGTFTPG GTFDSAIEKL 130 140 150 160 170 180 DYLVDLGVDF VEVMPVNAFS GTHGWGYDGV LWYAVHEPYG GPDGLVRFVD ACHARGLGVL 190 200 210 220 230 240 IDAVFNHLGP SGNYLPKFGP YLSSGSNPWG ESINIADAGA DEVRRYILDC ALRWMRDFHA 250 260 270 280 290 300 DGLRLDAVHA LVDTTAVHIL EELSAETDSL ATELGRPLSL VAESDMNDPR LITPRDQGGL 310 320 330 340 350 360 GMTAQWDDDI HHAIHSAVSG ERQGYYGDFG AVATLADTLR HGYFHAGTFS TFRGRRHGRP 370 380 390 400 410 420 LDTATIPATR LLAYTLTHDQ VGNRAIGDRP SQNLTTGQLA VKAALALGSP YTAMLFMGEE 430 440 450 460 470 480 WASSSPFQFF SSHPEPELAR ATAEGRKREF AEHGWDADEI PDPQDPATFE RSKLHWDEKD 490 500 510 520 530 540 GGDHARLLGF YRDLITLRRS ESDMADPWLD HLRVDYDEDA RWLVMHRGGL SIACNLGAEP 550 560 570 VDVPVAGDVV LAWDEPTVGN ETARLGGHSV AVLRRTG

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP46107.1.
RefSeq	YP_001134895.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T9K8.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T9K8.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000022445; EBMYCP00000022107; EBMYCG00000022440.
GeneID	4974951.
GenomeReviews	Gene locus Mflv_3633 in contig CP000656_GR.
KEGG	mgi:Mflv_3633.
PATRIC	18035436. VBIMycGil17082_3693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
GeneTree	EBGT00050000016657.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4T9K8_MYCGI

Accession

Primary (citable) accession number: A4T9K8

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)