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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei184SubstrateUniRule annotation1
Active sitei185Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Mflv_2065Imported
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))Imported
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_2065

Structurei

3D structure databases

ProteinModelPortaliA4T9A5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 340Lyase_1InterPro annotationAdd BLAST325
Domaini406 – 462FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 104Substrate bindingUniRule annotation3
Regioni126 – 129Substrate binding (B site)UniRule annotation4
Regioni136 – 138Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
KOiK01679
OMAiVSFTDNC
OrthoDBiPOG091H01XG

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

A4T9A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKDVEYRI EHDTMGEVRV PKDALWRAQT QRAVENFPIS FRPLERTQIR
60 70 80 90 100
AMGLLKGACA QVNKDLGLLA PEKADAIIAA AAEIADGRHD DQFPIDVFQT
110 120 130 140 150
GSGTSSNMNT NEVIASIAAA NGVTIHPNDD VNMSQSSNDT FPTATHIAAT
160 170 180 190 200
EAAVRHLIPA LEVLHESLDA KARQWRTTVK SGRTHLMDAV PVTLGQEFGG
210 220 230 240 250
YARQIEAGIE RVKATLPRLG ELAIGGTAVG TGLNAPDGFG AKVVEVLVDQ
260 270 280 290 300
TGIAELRTAK DSFEAQAARD GLVEASGALK TIAASLTKIA NDIRWMGSGP
310 320 330 340 350
LTGLGELQLP DLQPGSSIMP GKVNPVIPEA VTQVAAQVIG NDAAVTVGGL
360 370 380 390 400
SGAFELNVYI PMMARNVLES FTLLANVSKL FAAKCIDGLI ANDQHIREQA
410 420 430 440 450
ESSPSIVTPL NSAIGYEEAA KVAKQALKEK KTIRQTVIDR GLIGDKLSEE
460
ELDKRLDVLA MAKVKPGDQ
Length:469
Mass (Da):49,843
Last modified:May 15, 2007 - v1
Checksum:iB00DCEF425259CDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA Translation: ABP44543.1
RefSeqiWP_011892954.1, NC_009338.1

Genome annotation databases

EnsemblBacteriaiABP44543; ABP44543; Mflv_2065
KEGGimgi:Mflv_2065

Similar proteinsi

Entry informationi

Entry nameiA4T9A5_MYCGI
AccessioniPrimary (citable) accession number: A4T9A5
Entry historyiIntegrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: June 20, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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