A4T986 (ISPH_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Mflv_2079

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000077520

Sequences

	Sequence		Length	Mass (Da)
	A4T986 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 4CC39BEA0AD167DB	FASTA	333	35,844
	10 20 30 40 50 60 MPPTVNMGIP GAASSVVERT AGKRVLLAEP RGYCAGVDRA VETVERALEK HGAPIYVRHE 70 80 90 100 110 120 IVHNRYVVDT LAKAGAIFVE QTDEVPEGAI VVFSAHGVAP TVHVEAAARN LKTIDATCPL 130 140 150 160 170 180 VTKVHNEAKR FARDDYDILL VGHEGHEEVV GTAGEAPDHV QVVDNPDAVD NVVVRDPNKV 190 200 210 220 230 240 IWLSQTTLSV DETMETVRRL REKFPTLQDP PSDDICYATQ NRQVAVKAMA PECELVIVVG 250 260 270 280 290 300 SKNSSNSVRL VEVALGAGSR AAHLVDYAED IDPAWLDGVT TVGVTSGASV PEILVRGVLD 310 320 330 RLAEHGFDTV QPVTTANETL VFALPREIRP ARA

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP44557.1.
RefSeq	YP_001133345.1. NC_009338.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4T986.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000024491; EBMYCP00000024153; EBMYCG00000024486.
GeneID	4973401.
GenomeReviews	Gene locus Mflv_2079 in contig CP000656_GR.
KEGG	mgi:Mflv_2079.
PATRIC	18032261. VBIMycGil17082_2125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
GeneTree	EBGT00050000016858.
HOGENOM	HBG335228.
OMA	AMAPECD.
PhylomeDB	A4T986.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_2079-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_MYCGI

Accession

Primary (citable) accession number: A4T986

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program