A4T8Q2 (CYSD_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	Mflv_2271

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000092208

Sequences

	Sequence		Length	Mass (Da)
	A4T8Q2 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 0E4A5BCE9B3AF4D5	FASTA	309	35,356
	10 20 30 40 50 60 MTATDELTHN AGRYELSHLR ALEAEAIHII REVAAEFERP VLLFSGGKDS IVMLHLAVKA 70 80 90 100 110 120 FRPGRLPFPV MHVDTGHNFD EVLQARDELV AETGVRLVVA KVQDDIDAGR VVETIPSRNP 130 140 150 160 170 180 MQTFTLLRAI RENKFDAAFG GARRDEEKAR AKERVFSFRD EFGQWDPKNQ RPELWNLYNG 190 200 210 220 230 240 RHRKGEHIRA FPLSNWTEFD IWSYIGAEQI KLPSIYYAHE RKVFERDGML LAVHKYLQPR 250 260 270 280 290 300 KDEPIIEKTV RFRTVGDVTC TGCVESTAAT VSEVIAETAI SRLTERGATR ADDRISEAGM EDRKREGYF

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP44749.1.
RefSeq	YP_001133537.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T8Q2.
SMR	A4T8Q2. Positions 15-218.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T8Q2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000024412; EBMYCP00000024074; EBMYCG00000024407.
GeneID	4973593.
GenomeReviews	Gene locus Mflv_2271 in contig CP000656_GR.
KEGG	mgi:Mflv_2271.
PATRIC	18032657. VBIMycGil17082_2323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
GeneTree	EBGT00050000016335.
HOGENOM	HBG758022.
OMA	NYPEVIQ.
PhylomeDB	A4T8Q2.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_2271-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_MYCGI

Accession

Primary (citable) accession number: A4T8Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program