A4T8M4 (SYR_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Mflv_2296

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm By similarity HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Arginine--tRNA ligase HAMAP MF_00123		PRO_1000076222
Regions
	Motif	130 – 140	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	A4T8M4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A47BAA39AC88EA57	FASTA	550	59,204
	10 20 30 40 50 60 MTPADLAQLL KATAAAVLAE HGLDSAALPD TVTVERPRNP EHGDYATNLA LQLGKKVGAN 70 80 90 100 110 120 PRELAGWLAE ALAASDGIAA ADVAGPGFVN LRIEASAQNV MVSNVLSAGA DYGHSAVLAG 130 140 150 160 170 180 KKVNLEFVSA NPTGPIHIGG TRWAAVGDAL GRLLTTQGAA VTREYYFNDH GAQIDRFSNS 190 200 210 220 230 240 LIAAAKGEPA PEDGYAGTYI ADIASQVVAK EPGVLDQPDD RMRETFRAIG VNLMFDHIKE 250 260 270 280 290 300 SLHEFGTDFD VYTHEDSMHT SGRVEQAIDK LRETGFAYEK DGAVWLRTTD FGDDKDRVVI 310 320 330 340 350 360 KSDGKPAYIA GDLAYFLDKR KRGFDLCIYM LGADHHGYIA RLKASAAALG DDPDTVEVLI 370 380 390 400 410 420 GQMVNLVRDG QPVRMSKRAG TVITLDDLVE AIGVDAARYS LIRSSVDTPI DIDLELWSSA 430 440 450 460 470 480 SNENPVYYVQ YAHARLSALA RNAAELGIAP DTAHLDLLTH DKEGVLIRNI GEFPRILATA 490 500 510 520 530 540 AELREPHRVS RYLEDLAGDY HRFYDACRVL PQGDEAPGDL HAARLALCAA TRQVIANGLD 550 ILGVSAPERM

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP44774.1.
RefSeq	YP_001133562.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T8M4.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T8M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000024709; EBMYCP00000024371; EBMYCG00000024704.
GeneID	4973619.
GenomeReviews	Gene locus Mflv_2296 in contig CP000656_GR.
KEGG	mgi:Mflv_2296.
PATRIC	18032709. VBIMycGil17082_2348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
GeneTree	EBGT00050000016532.
HOGENOM	HBG695395.
OMA	FDVYFHE.
PhylomeDB	A4T8M4.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_2296-MONOMER.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_MYCGI

Accession

Primary (citable) accession number: A4T8M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program