A4T8H4 (A4T8H4_MYCGI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Mflv_2343

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	417	1	Nucleophile By similarity HAMAP MF_00685
	Active site	470	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A4T8H4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 67C67B31C7E82565	FASTA	741	83,544
	10 20 30 40 50 60 MTNASNLKNQ IDSPHLRPHT ADLNRLLAGE HHDPHSVLGA HEYDDHTVIR AYRPHAVSVE 70 80 90 100 110 120 ALIGGTRYPL RHIESGLFAV AVPFTGLIDY RLAIRYSDDE SAFVHTVADA YRFLPTLGEI 130 140 150 160 170 180 DLHLFGEGRH ERLWEILGAH RRTFTTADGE VTGVSFAVWA PNAKGVSVTG DFNHWGNEAQ 190 200 210 220 230 240 MRVLGSTGVW EVFWPNFPDG GLYKFRVHGA DGSVVDRADP MAFATEVPPQ TASKVFESEY 250 260 270 280 290 300 TWDDAEWMDG RTLRNPVFEP MSTYEVHLGS WRPGLSYTEL AEQLTEYLVQ HGFTHVEMLP 310 320 330 340 350 360 VAEHPFGGSW GYQVTSYYAP SSRFGSPDEF RYLVDALHRA GIGVLVDWVP AHFPKDSWAL 370 380 390 400 410 420 GRFDGTALYE HGDPRRGEQL DWGTYVFDFG RAEVRNFLVA NALYWLQEYH IDGLRVDAVA 430 440 450 460 470 480 SMLYLDYSRP EGGWSPNIYG GRENLEAVQF LQEMNATVHK INPGIVTIAE ESTSWPGVTR 490 500 510 520 530 540 PTNLGGLGFS MKWNMGWMND TLEFIKRDPI HRSFHHGEIT FSMIYAFSEN FVLPISHDEV 550 560 570 580 590 600 VHGKGTLWGR MPGNDHMKAA GIRSLLAYQW AHPGKQLLFM GQEFGQRAEW SEERGVDWFQ 610 620 630 640 650 660 LDEQGFSDGI LRMLSDANHI YRDRRALWSR DTQPEGYSWI DANDSANNVL SFLRFGDDGS 670 680 690 700 710 720 MMACVFNFSG AEHSRYRLGL PHAGTWREVL NTDSDTYHGA GIGNYGSVEA TDEPWHGRPA 730 740 SAVMVLPPLS ALWFEPVPPE A

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP44821.1.
RefSeq	YP_001133609.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T8H4.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T8H4.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000027472; EBMYCP00000027134; EBMYCG00000027467.
GeneID	4973665.
GenomeReviews	Gene locus Mflv_2343 in contig CP000656_GR.
KEGG	mgi:Mflv_2343.
PATRIC	18032809. VBIMycGil17082_2395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
GeneTree	EBGT00050000016657.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	A4T8H4.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4T8H4_MYCGI

Accession

Primary (citable) accession number: A4T8H4

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)