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A4T8B7 (MHPB_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16
Gene names
Name:mhpB
Ordered Locus Names:Mflv_2402
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653

Catalytic activity

3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653

(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653

Cofactor

Fe2+ ion By similarity. HAMAP MF_01653

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653

Subunit structure

Homotetramer By similarity. HAMAP MF_01653

Sequence similarities

Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function3-carboxyethylcatechol 2,3-dioxygenase activity

Inferred from electronic annotation. Source: EC

ferrous iron binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3153152,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653
PRO_0000337653

Sites

Active site1181Proton donor By similarity
Active site1821Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A4T8B7 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: F17F6AB3954C0398

FASTA31533,369
        10         20         30         40         50         60 
MAKSQIALCC MSHSPLLNLP GPAQELLDEI DKAIAAARDF VAEFDPELVV TFSPDHYNGF 

        70         80         90        100        110        120 
FYRAMPPFCI GTAAEGVGDY GTHEGPLDVP SDLATDCARA VLDHDVDVAL SAAMDVDHGT 

       130        140        150        160        170        180 
VQPLQKLFGD ATAKPVIPVF INSVATPLGP IRRVRALGAA VGAHLATLGK RVLVIGSGGL 

       190        200        210        220        230        240 
SHDPPVPTLA TAPPAALDRI VRGVPMTTDQ RQARQTAVIE AAREFASGRG TLAPLNPDWD 

       250        260        270        280        290        300 
RAFLDIVDSG RLAEVDGWDN GWIAEQAGNS AHEVRTWIAA FAALAAQGEY VTENRFYRAA 

       310 
PELIAGFAIR TAVTT

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PYR-GCK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000656 Genomic DNA. Translation: ABP44879.1.
RefSeqYP_001133667.1. NC_009338.1.

3D structure databases

ProteinModelPortalA4T8B7.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4T8B7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000026143; EBMYCP00000025805; EBMYCG00000026138.
GeneID4973723.
GenomeReviewsGene locus Mflv_2402 in contig CP000656_GR.
KEGGmgi:Mflv_2402.
PATRIC18032929. VBIMycGil17082_2454.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG42398.
GeneTreeEBGT00050000017557.
HOGENOMHBG565586.
OMAAHEIRTW.
ProtClustDBPRK13370.

Enzyme and pathway databases

BioCycMGIL350054:MFLV_2402-MONOMER.

Family and domain databases

HAMAPMF_01653. MhpB.
[Tree]
InterProIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
Gene3DG3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KOK05713.
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMHPB_MYCGI
AccessionPrimary (citable) accession number: A4T8B7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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