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A4T7Q6 (OTSA_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-
Alternative name(s):
Trehalose-6-phosphate synthase
Gene names
Name:otsA
Ordered Locus Names:Mflv_1566
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.

Catalytic activity

Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtrehalose biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Trehalose-phosphate synthase
PRO_0000348906

Regions

Region51 – 522NDP-glucose binding By similarity
Region408 – 4125NDP-glucose binding By similarity

Sites

Binding site311Glucose-6-phosphate By similarity
Binding site1091Glucose-6-phosphate By similarity
Binding site1631Glucose-6-phosphate By similarity
Binding site3051NDP-glucose By similarity
Binding site3101NDP-glucose By similarity
Binding site3431Glucose-6-phosphate By similarity
Site1181Involved in alpha anomer selectivity By similarity
Site1881Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
A4T7Q6 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: CA212EB6FBC86585

FASTA50356,374
        10         20         30         40         50         60 
MTDQSGNGVR SGSASEAPPS AKADFVVVAN RLPIDQVNLP DGSTEWKRSP GGLVTALEPL 

        70         80         90        100        110        120 
LRRRHGAWIG WPGVPEDADD PDASTEPIEQ DGMTLVPVRL SAADVAKYYE GFSNATLWPL 

       130        140        150        160        170        180 
YHDVIVKPTY HREWWERYVE VNRRFAEAAA RTAAEGATVW VQDYQLQLVP KMLRMLRPDL 

       190        200        210        220        230        240 
TIGFFLHIPF PPVELFLQMP WRTEIIEGLL GADLVGFHLP GGAQNFLILA RRLLGAVTSR 

       250        260        270        280        290        300 
GNVGVRSRFG EVQFGFRKVK VGAFPISIDS AELDQHARTR ATRQRAKEIR AELGNPRKVL 

       310        320        330        340        350        360 
LGVDRLDYTK GIDVRLRAFS ELLEEGRIDP EDTVLVQLAT PSRERVQSYI EMREDIERQV 

       370        380        390        400        410        420 
GHVNGEFGKV GHPVLHYLHR PIPREDLVAF FVAADVMLVT PLRDGMNLVA KEYVACRHDL 

       430        440        450        460        470        480 
GGALVLSEFT GAAAELRQAY LTNPHHLEGV KDAIEAALNQ SPEEGRRRMR ALRRQVLAHD 

       490        500 
VDRWARSFLD ALASREPINE ESH

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PYR-GCK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000656 Genomic DNA. Translation: ABP44048.1.
RefSeqYP_001132836.1. NC_009338.1.

3D structure databases

ProteinModelPortalA4T7Q6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4T7Q6.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000023843; EBMYCP00000023505; EBMYCG00000023838.
GeneID4972892.
GenomeReviewsGene locus Mflv_1566 in contig CP000656_GR.
KEGGmgi:Mflv_1566.
PATRIC18031195. VBIMycGil17082_1602.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0380.
GeneTreeEBGT00050000016525.
HOGENOMHBG559076.
OMANDLARYE.
PhylomeDBA4T7Q6.
ProtClustDBCLSK872185.

Enzyme and pathway databases

BioCycMGIL350054:MFLV_1566-MONOMER.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
[Graphical view]
KOK00697.
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTSA_MYCGI
AccessionPrimary (citable) accession number: A4T7Q6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 15, 2007
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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