A4T5R5 (A4T5R5_MYCGI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mflv_1394

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	941 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A4T5R5 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 696C7A3D2A1CDFBA	FASTA	941	102,965
	10 20 30 40 50 60 MADGDRGSGK NGSVRRLVIV ESPTKARKIA GYLGSNYVVE SSRGHIRDLP RAAADVPAKY 70 80 90 100 110 120 KSEPWARLGV NVDADFEPLY IISPDKKATV ADLKDKLKNV DELYLATDGD REGEAIAWHL 130 140 150 160 170 180 LETLKPRIPV KRMVFHEITE PAIRAAAEDP RDLDNDLVDA QETRRILDRL YGYEVSPVLW 190 200 210 220 230 240 KKVAPKLSAG RVQSVATRII VQRERERMAF RSAGYWDVTA ELDASVSDAQ ATPPTFVAKL 250 260 270 280 290 300 NTVDGRRVAA GRDFDSLGAV KKPGEVLVLD EAAANALAGG LRGAQLSVSS VEQKPYTRRP 310 320 330 340 350 360 YAPFMTSTLQ QEAGRKLRMS SERTMSIAQR LYENGYITYM RTDSTTLSQS AIDAARNQAR 370 380 390 400 410 420 QLYGEEYVHP TPRQYTRKVK NAQEAHEAIR PAGDVFQTPG QLHSQLDTDE FRLYELIWQR 430 440 450 460 470 480 TVASQMADAR GTTLSLRIAG AATSGEQVVF NASGRTITFA GFLKAYVESL DEQAGGEADD 490 500 510 520 530 540 AESRLPNLTQ GQRVDAKDLT ADGHTTSPPA RYTEASLIKA LEDLGIGRPS TYSSIIKTIQ 550 560 570 580 590 600 DRGYVHKKGS ALVPSWVAFA VIGLLEQHFS RLVDYDFTAA MEDELDEIAA GNERRTNWLN 610 620 630 640 650 660 NFYFGGEHGV EGSIAREGGL KKLVGGNLEE IDAREVNSIK LFDDSEGRAV NVRVGRNGAY 670 680 690 700 710 720 LERMVADPDN PGELKPQRAN LKDELTPDEL TLELAEKLFA TPQEGRSLGI DPATGHEIVA 730 740 750 760 770 780 KDGRYGPYVT EVLPEPPDDG EAGATAKKGK KPTGPKPRTG SLLRSMDLET VTLDDALRLL 790 800 810 820 830 840 SLPRVVGVDP ANGEEITAQN GRYGPYLKRG TDSRSLATEE QMFDITLEEA LKIYAEPKRR 850 860 870 880 890 900 GRQGAATPPL RELGVDPVSE KPMVIKDGRF GPYVTDGETN ASLRKGDDVM SITDARASEL 910 920 930 940 LADRRARGPV KKKAAKKAAV KKTAAKKAAK KAPAKKAAKK A

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP43876.1.
RefSeq	YP_001132664.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T5R5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T5R5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000026401; EBMYCP00000026063; EBMYCG00000026396.
GeneID	4972720.
GenomeReviews	Gene locus Mflv_1394 in contig CP000656_GR.
KEGG	mgi:Mflv_1394.
PATRIC	18030838. VBIMycGil17082_1425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4T5R5_MYCGI

Accession

Primary (citable) accession number: A4T5R5

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)