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A4T4R2 (SYL_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mflv_0853
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length949 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 949949Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334774

Regions

Motif68 – 7912"HIGH" region HAMAP-Rule MF_00049
Motif722 – 7265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4T4R2 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 84E6A5DB25C6AF7E

FASTA949105,013
        10         20         30         40         50         60 
MTETPIAPLD DTPRFRYTAV LAGEIERAWQ QQWADSGTFH VDNPVGSLAP ADGSAVPADK 

        70         80         90        100        110        120 
MFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYYRMTGRNV LHALGFDAFG LPAEQYAIQT 

       130        140        150        160        170        180 
GTHPRTRTEA NIVNFRRQLG RLGLGHDSRR SFATTDVDYY KWTQWIFLQI FNAWFDTDQN 

       190        200        210        220        230        240 
RARPIRELIA EFEAGTRQVG DGRSWADLDA GARADLVDAH RLVYLADSVV NWCPGLGTVL 

       250        260        270        280        290        300 
ANEEVTADGR SERGNFPVFR KRLRQWMMRI TAYSDRLLED LDVLDWPDKV KTMQRNWIGR 

       310        320        330        340        350        360 
STGAEVQFST AAGDIEVFTT RPDTLFGATY MVLAPEHDLV DRLVASAWPD GTDARWTFGA 

       370        380        390        400        410        420 
ATPAEAVAAY RAGIAAKSDL ERQENKTKTG VFLGAYATNP ANGQQVPVFI ADYVLAGYGT 

       430        440        450        460        470        480 
GAIMAVPSGD QRDWDFATEF GLPIVEVVAG GDVTVEAYSG DGTMVNSGFL DGMDVATAKQ 

       490        500        510        520        530        540 
AMTERLVADG RGRARVEYKL RDWLFARQRY WGEPFPVVYD SEGRAHGLPE GMLPVELPDV 

       550        560        570        580        590        600 
PDYSPVSFDP DDAGSEPSPP LGKVTDWVNV DLDLGDGLKP YTRDTNVMPQ WAGSSWYELR 

       610        620        630        640        650        660 
YTDPYNSEAL CAKENEAYWM GPRPAEHGPD DPGGVDLYVG GVEHAVLHLL YSRFWHKVLY 

       670        680        690        700        710        720 
DLGHVSSREP YRRLVNQGYI QAFAYTDSRG SYVPAAEVIE RDGKFVWPGP DGETEVNQEF 

       730        740        750        760        770        780 
GKIGKSLKNS VSPDEICDNY GADTLRVYEM SMGPLEASRP WATKDVVGAY RFLQRVWRLV 

       790        800        810        820        830        840 
VDENTGETLA TEDALDDDTL RLLHRTIAGT ADDYASLRNN TAAAKLIEYT NHLTKQSVTA 

       850        860        870        880        890        900 
RAALEPLVLM VAPLAPHLAE ELWKRLGHEG SLAHGPFPLA DERYLVEDTV EYPVQVNGKV 

       910        920        930        940 
RGRVTVPADA PADAVEAAAL AEEKVVAFLD GRTPKKVIVV AGRLVNVVV 

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References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PYR-GCK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000656 Genomic DNA. Translation: ABP43337.1.
RefSeqYP_001132125.1. NC_009338.1.

3D structure databases

ProteinModelPortalA4T4R2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350054.Mflv_0853.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP43337; ABP43337; Mflv_0853.
GeneID4972181.
KEGGmgi:Mflv_0853.
PATRIC18029721. VBIMycGil17082_0872.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMGIL350054:GHK8-857-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCGI
AccessionPrimary (citable) accession number: A4T4R2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries