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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei725ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mflv_0853
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003347741 – 949Leucine--tRNA ligaseAdd BLAST949

Interactioni

Protein-protein interaction databases

STRINGi350054.Mflv_0853.

Structurei

3D structure databases

ProteinModelPortaliA4T4R2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 79"HIGH" regionAdd BLAST12
Motifi722 – 726"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4T4R2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETPIAPLD DTPRFRYTAV LAGEIERAWQ QQWADSGTFH VDNPVGSLAP
60 70 80 90 100
ADGSAVPADK MFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYYRMTGRNV
110 120 130 140 150
LHALGFDAFG LPAEQYAIQT GTHPRTRTEA NIVNFRRQLG RLGLGHDSRR
160 170 180 190 200
SFATTDVDYY KWTQWIFLQI FNAWFDTDQN RARPIRELIA EFEAGTRQVG
210 220 230 240 250
DGRSWADLDA GARADLVDAH RLVYLADSVV NWCPGLGTVL ANEEVTADGR
260 270 280 290 300
SERGNFPVFR KRLRQWMMRI TAYSDRLLED LDVLDWPDKV KTMQRNWIGR
310 320 330 340 350
STGAEVQFST AAGDIEVFTT RPDTLFGATY MVLAPEHDLV DRLVASAWPD
360 370 380 390 400
GTDARWTFGA ATPAEAVAAY RAGIAAKSDL ERQENKTKTG VFLGAYATNP
410 420 430 440 450
ANGQQVPVFI ADYVLAGYGT GAIMAVPSGD QRDWDFATEF GLPIVEVVAG
460 470 480 490 500
GDVTVEAYSG DGTMVNSGFL DGMDVATAKQ AMTERLVADG RGRARVEYKL
510 520 530 540 550
RDWLFARQRY WGEPFPVVYD SEGRAHGLPE GMLPVELPDV PDYSPVSFDP
560 570 580 590 600
DDAGSEPSPP LGKVTDWVNV DLDLGDGLKP YTRDTNVMPQ WAGSSWYELR
610 620 630 640 650
YTDPYNSEAL CAKENEAYWM GPRPAEHGPD DPGGVDLYVG GVEHAVLHLL
660 670 680 690 700
YSRFWHKVLY DLGHVSSREP YRRLVNQGYI QAFAYTDSRG SYVPAAEVIE
710 720 730 740 750
RDGKFVWPGP DGETEVNQEF GKIGKSLKNS VSPDEICDNY GADTLRVYEM
760 770 780 790 800
SMGPLEASRP WATKDVVGAY RFLQRVWRLV VDENTGETLA TEDALDDDTL
810 820 830 840 850
RLLHRTIAGT ADDYASLRNN TAAAKLIEYT NHLTKQSVTA RAALEPLVLM
860 870 880 890 900
VAPLAPHLAE ELWKRLGHEG SLAHGPFPLA DERYLVEDTV EYPVQVNGKV
910 920 930 940
RGRVTVPADA PADAVEAAAL AEEKVVAFLD GRTPKKVIVV AGRLVNVVV
Length:949
Mass (Da):105,013
Last modified:May 15, 2007 - v1
Checksum:i84E6A5DB25C6AF7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP43337.1.
RefSeqiWP_011891757.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP43337; ABP43337; Mflv_0853.
KEGGimgi:Mflv_0853.
PATRICi18029721. VBIMycGil17082_0872.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP43337.1.
RefSeqiWP_011891757.1. NC_009338.1.

3D structure databases

ProteinModelPortaliA4T4R2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_0853.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP43337; ABP43337; Mflv_0853.
KEGGimgi:Mflv_0853.
PATRICi18029721. VBIMycGil17082_0872.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_MYCGI
AccessioniPrimary (citable) accession number: A4T4R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.