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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Mflv_1124

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Metal bindingi101Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Mflv_1124
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000812091 – 160Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST160

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi350054.Mflv_1124.

Structurei

3D structure databases

ProteinModelPortaliA4T3Z0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 81Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiVYADWNG.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

A4T3Z0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTPRPTAD LVDEIGPEVR SCDLQMRQFG GRSEFAGPIT TVRCFEDNAL
60 70 80 90 100
LKSVLSEPGD GGVLVVDGDG SVHTALVGDV IAELGRSNGW SGLIINGAVR
110 120 130 140 150
DASTLRTLDI GIKALGTNPR KSSKTGDGVR DAVVEFGGVV FTPGDIAYSD
160
DDGIVVVTAD
Length:160
Mass (Da):16,574
Last modified:May 15, 2007 - v1
Checksum:i8DD6CB47D82ADBF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP43606.1.
RefSeqiWP_011892023.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP43606; ABP43606; Mflv_1124.
KEGGimgi:Mflv_1124.
PATRICi18030274. VBIMycGil17082_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP43606.1.
RefSeqiWP_011892023.1. NC_009338.1.

3D structure databases

ProteinModelPortaliA4T3Z0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_1124.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP43606; ABP43606; Mflv_1124.
KEGGimgi:Mflv_1124.
PATRICi18030274. VBIMycGil17082_1149.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiVYADWNG.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_MYCGI
AccessioniPrimary (citable) accession number: A4T3Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.