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A4T3S4

- TRMB_MYCGI

UniProt

A4T3S4 - TRMB_MYCGI

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
    Binding sitei114 – 1141S-adenosyl-L-methionineUniRule annotation
    Binding sitei146 – 1461S-adenosyl-L-methionineUniRule annotation
    Active sitei169 – 1691By similarity
    Binding sitei169 – 1691S-adenosyl-L-methionineUniRule annotation
    Binding sitei173 – 1731SubstrateUniRule annotation
    Binding sitei205 – 2051SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMGIL350054:GHK8-455-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:Mflv_0454
    OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
    Taxonomic identifieri350054 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000232: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263tRNA (guanine-N(7)-)-methyltransferasePRO_1000084445Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi350054.Mflv_0454.

    Structurei

    3D structure databases

    ProteinModelPortaliA4T3S4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni242 – 2454Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4T3S4-1 [UniParc]FASTAAdd to Basket

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    MSDHGRMHST GSEVAAPVAP DPDTEGVHPH FNRRVTSFRA RRSTISDGQQ    50
    ATWDRLWPQL GRQARDGDEP PGPLDTDAWF GRHAPVVLEI GCGTGTSTLA 100
    MAQAEPGIDV VAVEVYRRGL AQLLSAIDRT AATEHPVSNI RLIRGDGVDV 150
    LTHMFGSASL TGVRVFFPDP WPKARHHKRR LLQPDTMALI ADRLRPGGVL 200
    HAATDHQGYA AHIAEVGDAE PRLRRVAMDS ADLPMSVTRP VTKYERKALA 250
    GPVVTELLWE RTP 263
    Length:263
    Mass (Da):28,766
    Last modified:May 15, 2007 - v1
    Checksum:i001B73924B864736
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP42948.1.
    RefSeqiYP_001131736.1. NC_009338.1.

    Genome annotation databases

    EnsemblBacteriaiABP42948; ABP42948; Mflv_0454.
    GeneIDi4971530.
    KEGGimgi:Mflv_0454.
    PATRICi18028916. VBIMycGil17082_0472.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP42948.1 .
    RefSeqi YP_001131736.1. NC_009338.1.

    3D structure databases

    ProteinModelPortali A4T3S4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350054.Mflv_0454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP42948 ; ABP42948 ; Mflv_0454 .
    GeneIDi 4971530.
    KEGGi mgi:Mflv_0454.
    PATRICi 18028916. VBIMycGil17082_0472.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci MGIL350054:GHK8-455-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PYR-GCK.

    Entry informationi

    Entry nameiTRMB_MYCGI
    AccessioniPrimary (citable) accession number: A4T3S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3