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A4T3S4

- TRMB_MYCGI

UniProt

A4T3S4 - TRMB_MYCGI

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
Binding sitei114 – 1141S-adenosyl-L-methionineUniRule annotation
Binding sitei146 – 1461S-adenosyl-L-methionineUniRule annotation
Active sitei169 – 1691By similarity
Binding sitei169 – 1691S-adenosyl-L-methionineUniRule annotation
Binding sitei173 – 1731SubstrateUniRule annotation
Binding sitei205 – 2051SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-455-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Mflv_0454
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263tRNA (guanine-N(7)-)-methyltransferasePRO_1000084445Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi350054.Mflv_0454.

Structurei

3D structure databases

ProteinModelPortaliA4T3S4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 2454Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4T3S4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDHGRMHST GSEVAAPVAP DPDTEGVHPH FNRRVTSFRA RRSTISDGQQ
60 70 80 90 100
ATWDRLWPQL GRQARDGDEP PGPLDTDAWF GRHAPVVLEI GCGTGTSTLA
110 120 130 140 150
MAQAEPGIDV VAVEVYRRGL AQLLSAIDRT AATEHPVSNI RLIRGDGVDV
160 170 180 190 200
LTHMFGSASL TGVRVFFPDP WPKARHHKRR LLQPDTMALI ADRLRPGGVL
210 220 230 240 250
HAATDHQGYA AHIAEVGDAE PRLRRVAMDS ADLPMSVTRP VTKYERKALA
260
GPVVTELLWE RTP
Length:263
Mass (Da):28,766
Last modified:May 15, 2007 - v1
Checksum:i001B73924B864736
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP42948.1.
RefSeqiWP_011891455.1. NC_009338.1.
YP_001131736.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP42948; ABP42948; Mflv_0454.
GeneIDi4971530.
KEGGimgi:Mflv_0454.
PATRICi18028916. VBIMycGil17082_0472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP42948.1 .
RefSeqi WP_011891455.1. NC_009338.1.
YP_001131736.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4T3S4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_0454.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP42948 ; ABP42948 ; Mflv_0454 .
GeneIDi 4971530.
KEGGi mgi:Mflv_0454.
PATRICi 18028916. VBIMycGil17082_0472.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci MGIL350054:GHK8-455-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiTRMB_MYCGI
AccessioniPrimary (citable) accession number: A4T3S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3