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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89S-adenosyl-L-methionineUniRule annotation1
Binding sitei114S-adenosyl-L-methionineUniRule annotation1
Binding sitei146S-adenosyl-L-methionineUniRule annotation1
Active sitei169By similarity1
Binding sitei169S-adenosyl-L-methionineUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Binding sitei205SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Mflv_0454
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000844451 – 263tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST263

Interactioni

Protein-protein interaction databases

STRINGi350054.Mflv_0454

Structurei

3D structure databases

ProteinModelPortaliA4T3S4
SMRiA4T3S4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 245Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A4T3S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDHGRMHST GSEVAAPVAP DPDTEGVHPH FNRRVTSFRA RRSTISDGQQ
60 70 80 90 100
ATWDRLWPQL GRQARDGDEP PGPLDTDAWF GRHAPVVLEI GCGTGTSTLA
110 120 130 140 150
MAQAEPGIDV VAVEVYRRGL AQLLSAIDRT AATEHPVSNI RLIRGDGVDV
160 170 180 190 200
LTHMFGSASL TGVRVFFPDP WPKARHHKRR LLQPDTMALI ADRLRPGGVL
210 220 230 240 250
HAATDHQGYA AHIAEVGDAE PRLRRVAMDS ADLPMSVTRP VTKYERKALA
260
GPVVTELLWE RTP
Length:263
Mass (Da):28,766
Last modified:May 15, 2007 - v1
Checksum:i001B73924B864736
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA Translation: ABP42948.1
RefSeqiWP_011891455.1, NC_009338.1

Genome annotation databases

EnsemblBacteriaiABP42948; ABP42948; Mflv_0454
KEGGimgi:Mflv_0454

Similar proteinsi

Entry informationi

Entry nameiTRMB_MYCGI
AccessioniPrimary (citable) accession number: A4T3S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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