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A4T3S1

- PCKG_MYCGI

UniProt

A4T3S1 - PCKG_MYCGI

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
    Metal bindingi229 – 2291ManganeseUniRule annotation
    Binding sitei229 – 2291SubstrateUniRule annotation
    Metal bindingi249 – 2491Manganese; via tele nitrogenUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Active sitei273 – 2731UniRule annotation
    Metal bindingi296 – 2961ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi272 – 2776GTPUniRule annotation
    Nucleotide bindingi515 – 5184GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGIL350054:GHK8-452-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Mflv_0451
    OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
    Taxonomic identifieri350054 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000232: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080986Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi350054.Mflv_0451.

    Structurei

    3D structure databases

    ProteinModelPortaliA4T3S1.
    SMRiA4T3S1. Positions 12-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiIDWHGND.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4T3S1-1 [UniParc]FASTAAdd to Basket

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    MTAATIPGLD SAPTKHEGLL AWVREVAELT QPDRVAWADG SEEEYERLCA    50
    HLVEAGTFQK LNPDKQPNSY LALSDPSDVA RVESRTYICS EREIDAGPTN 100
    NWMDPAEMRG IMTDLYRGSM RGRTLWVVPF CMGPLNAEDP KLGVELTDSE 150
    YVVVSMRTMT RMGAAALEKI GDDGFFVKAL HSIGAPLEPG QADVPWPCND 200
    TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM 250
    LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM 300
    RFGKDGRLYA TNPEFGFFGV APGTNWDSNP NAMKTIAAGN TVFTNVAKTD 350
    DGDVWWEGLE GEPDHLIDWK GNDWVLRETE TKAAHPNSRY CTPISQCPTL 400
    APEWDDPQGV PISAILFGGR RKTTVPLITE ARDWQHGVFI GATLGSEQTA 450
    AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH WINIGKQADE SQLPKVFFVN 500
    WFRRGDDGRF LWPGFGENSR VLKWAVERIE HKADGKSTPI GIVPTAADLD 550
    LSGLDVNAAD VDEALDVKVD EWRAELPLIE EWFEFVGEKL PTGIKDEFDA 600
    LKTRLAEES 609
    Length:609
    Mass (Da):67,486
    Last modified:May 15, 2007 - v1
    Checksum:iA76D9ACD8D83E9F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP42945.1.
    RefSeqiYP_001131733.1. NC_009338.1.

    Genome annotation databases

    EnsemblBacteriaiABP42945; ABP42945; Mflv_0451.
    GeneIDi4971527.
    KEGGimgi:Mflv_0451.
    PATRICi18028910. VBIMycGil17082_0469.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP42945.1 .
    RefSeqi YP_001131733.1. NC_009338.1.

    3D structure databases

    ProteinModelPortali A4T3S1.
    SMRi A4T3S1. Positions 12-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350054.Mflv_0451.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP42945 ; ABP42945 ; Mflv_0451 .
    GeneIDi 4971527.
    KEGGi mgi:Mflv_0451.
    PATRICi 18028910. VBIMycGil17082_0469.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi IDWHGND.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci MGIL350054:GHK8-452-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PYR-GCK.

    Entry informationi

    Entry nameiPCKG_MYCGI
    AccessioniPrimary (citable) accession number: A4T3S1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3