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A4T3S1

- PCKG_MYCGI

UniProt

A4T3S1 - PCKG_MYCGI

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, Mflv_0451
Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811Substrate By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Metal bindingi229 – 2291Manganese By similarity
Binding sitei229 – 2291Substrate By similarity
Metal bindingi249 – 2491Manganese; via tele nitrogen By similarity
Binding sitei271 – 2711Substrate By similarity
Active sitei273 – 2731 By similarity
Metal bindingi296 – 2961Manganese By similarity
Binding sitei389 – 3891GTP By similarity
Binding sitei420 – 4201GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776GTP By similarity
Nucleotide bindingi515 – 5184GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-452-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:Mflv_0451
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000080986Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_0451.

Structurei

3D structure databases

ProteinModelPortaliA4T3S1.
SMRiA4T3S1. Positions 12-606.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiIDWHGND.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4T3S1-1 [UniParc]FASTAAdd to Basket

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MTAATIPGLD SAPTKHEGLL AWVREVAELT QPDRVAWADG SEEEYERLCA    50
HLVEAGTFQK LNPDKQPNSY LALSDPSDVA RVESRTYICS EREIDAGPTN 100
NWMDPAEMRG IMTDLYRGSM RGRTLWVVPF CMGPLNAEDP KLGVELTDSE 150
YVVVSMRTMT RMGAAALEKI GDDGFFVKAL HSIGAPLEPG QADVPWPCND 200
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM 250
LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM 300
RFGKDGRLYA TNPEFGFFGV APGTNWDSNP NAMKTIAAGN TVFTNVAKTD 350
DGDVWWEGLE GEPDHLIDWK GNDWVLRETE TKAAHPNSRY CTPISQCPTL 400
APEWDDPQGV PISAILFGGR RKTTVPLITE ARDWQHGVFI GATLGSEQTA 450
AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH WINIGKQADE SQLPKVFFVN 500
WFRRGDDGRF LWPGFGENSR VLKWAVERIE HKADGKSTPI GIVPTAADLD 550
LSGLDVNAAD VDEALDVKVD EWRAELPLIE EWFEFVGEKL PTGIKDEFDA 600
LKTRLAEES 609
Length:609
Mass (Da):67,486
Last modified:May 15, 2007 - v1
Checksum:iA76D9ACD8D83E9F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP42945.1.
RefSeqiYP_001131733.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP42945; ABP42945; Mflv_0451.
GeneIDi4971527.
KEGGimgi:Mflv_0451.
PATRICi18028910. VBIMycGil17082_0469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP42945.1 .
RefSeqi YP_001131733.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4T3S1.
SMRi A4T3S1. Positions 12-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_0451.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP42945 ; ABP42945 ; Mflv_0451 .
GeneIDi 4971527.
KEGGi mgi:Mflv_0451.
PATRICi 18028910. VBIMycGil17082_0469.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi IDWHGND.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci MGIL350054:GHK8-452-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiPCKG_MYCGI
AccessioniPrimary (citable) accession number: A4T3S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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