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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 2611D-inositol 3-phosphateUniRule annotation
Binding sitei40 – 401UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei95 – 9511D-inositol 3-phosphateUniRule annotation
Binding sitei128 – 12811D-inositol 3-phosphateUniRule annotation
Binding sitei152 – 15211D-inositol 3-phosphateUniRule annotation
Binding sitei172 – 17211D-inositol 3-phosphateUniRule annotation
Binding sitei246 – 2461UDP-GlcNAcUniRule annotation
Binding sitei251 – 2511UDP-GlcNAcUniRule annotation
Binding sitei304 – 3041UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi313 – 3131Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi314 – 3141Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi316 – 3161Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei326 – 3261UDP-GlcNAcUniRule annotation
Binding sitei334 – 3341UDP-GlcNAcUniRule annotation
Metal bindingi340 – 3401MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-86-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Mflv_0086
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442D-inositol 3-phosphate glycosyltransferasePRO_0000400134Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_0086.

Structurei

3D structure databases

ProteinModelPortaliA4T324.
SMRiA4T324. Positions 38-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 332UDP-GlcNAc bindingUniRule annotation
Regioni37 – 4261D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4T324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLATDPAGS SSGLPQPRRV AVLSVHTSPL AQPGTGDAGG MNVYVLQTSL
60 70 80 90 100
ELARRGVEVE IFTRATSSAD QPIVSVAPGV VVRNVVAGPF EGLDKNDLPT
110 120 130 140 150
QLCAFTAGVL RAEATHEPGY YDILHSHYWL SGQVGWLAAD RWAVPLVHTA
160 170 180 190 200
HTLAAVKNAS LAAGDTPEPP MRAIGEQQVV DEADRLIVNT EHEAQQLVSL
210 220 230 240 250
HHADPGRIDV VHPGVDLATF TPGDRAAARA ALGLDPAARI VAFVGRIQPL
260 270 280 290 300
KAPDVLLRAA ALLPDVHVVI AGGPSGSGMA TPDNLVHLAG ELGIAERVTF
310 320 330 340 350
LPPQSRDHLV RVYRAADIVA VPSHNESFGL VAVEAQACGT PVVAAAVGGL
360 370 380 390 400
PVAVRDGVSG ALVHSHEPDA WATTLGEVFA ADPSTFGRAA VDHAATFSWA
410 420 430 440
HTVDALLTGY GRAIADHRAD NTLQVAARRS GRRFSMRRGV RA
Length:442
Mass (Da):46,248
Last modified:May 15, 2007 - v1
Checksum:i5CE7037AF89661EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP42582.1.
RefSeqiWP_011891089.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP42582; ABP42582; Mflv_0086.
KEGGimgi:Mflv_0086.
PATRICi18028148. VBIMycGil17082_0088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP42582.1.
RefSeqiWP_011891089.1. NC_009338.1.

3D structure databases

ProteinModelPortaliA4T324.
SMRiA4T324. Positions 38-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_0086.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP42582; ABP42582; Mflv_0086.
KEGGimgi:Mflv_0086.
PATRICi18028148. VBIMycGil17082_0088.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-86-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCGI
AccessioniPrimary (citable) accession number: A4T324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.