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A4T2J5

- PROB_MYCGI

UniProt

A4T2J5 - PROB_MYCGI

Protein

Glutamate 5-kinase

Gene

proB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171ATPUniRule annotation
    Binding sitei57 – 571SubstrateUniRule annotation
    Binding sitei144 – 1441SubstrateUniRule annotation
    Binding sitei156 – 1561Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi176 – 1772ATPUniRule annotation
    Nucleotide bindingi220 – 2267ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGIL350054:GHK8-2669-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:Mflv_2638
    OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
    Taxonomic identifieri350054 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000232: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Glutamate 5-kinasePRO_1000081075Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi350054.Mflv_2638.

    Structurei

    3D structure databases

    ProteinModelPortaliA4T2J5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini282 – 36079PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    HOGENOMiHOG000246368.
    KOiK00931.
    OMAiFDAKEIP.
    OrthoDBiEOG6PGK7G.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4T2J5-1 [UniParc]FASTAAdd to Basket

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    MSVHRDAIRG ARSVVVKIGT TALTTPSGVF DRGRLHYLAD AIEARMKAGS    50
    DVVIVSSGAI AAGIEPLKLT KRPADLATKQ AAASVGQVAL VNSWSAAFGR 100
    YDRTVGQVLL TAHDISMRVQ HNNAQRTLDR LRALHAVAIV NENDTVATNE 150
    IRFGDNDRLS ALVAHLVGAD ALILLSDIDG LYDGDPRKAP EDNPARFISE 200
    VSGPDDLADV TAGRGSHLGT GGMVSKLSSA LLAADAGVPV LLAAAADAAS 250
    ALGDASVGTV FAPRPERMSA RRFWVRYAAE ASGTLTLDDG AVRAVVQQRR 300
    SLLPAGISAV SGRFFGGDVV ELRDQENAMV ARGVVAYDKA ELATMLGRST 350
    SDLPAEMRRP AVHADDLVAV 370
    Length:370
    Mass (Da):38,743
    Last modified:May 15, 2007 - v1
    Checksum:i066DAE52599D4F14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP45115.1.
    RefSeqiWP_011893518.1. NC_009338.1.
    YP_001133903.1. NC_009338.1.

    Genome annotation databases

    EnsemblBacteriaiABP45115; ABP45115; Mflv_2638.
    GeneIDi4973959.
    KEGGimgi:Mflv_2638.
    PATRICi18033425. VBIMycGil17082_2697.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP45115.1 .
    RefSeqi WP_011893518.1. NC_009338.1.
    YP_001133903.1. NC_009338.1.

    3D structure databases

    ProteinModelPortali A4T2J5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350054.Mflv_2638.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP45115 ; ABP45115 ; Mflv_2638 .
    GeneIDi 4973959.
    KEGGi mgi:Mflv_2638.
    PATRICi 18033425. VBIMycGil17082_2697.

    Phylogenomic databases

    eggNOGi COG0263.
    HOGENOMi HOG000246368.
    KOi K00931.
    OMAi FDAKEIP.
    OrthoDBi EOG6PGK7G.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci MGIL350054:GHK8-2669-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PYR-GCK.

    Entry informationi

    Entry nameiPROB_MYCGI
    AccessioniPrimary (citable) accession number: A4T2J5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3