A4T2I0 (PROA_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mflv_2644

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000080487

Sequences

	Sequence		Length	Mass (Da)
	A4T2I0 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A30346DB33A4282A	FASTA	415	43,448
	10 20 30 40 50 60 MSVQAPSVPD LRQQVHDAAR RARSAARGLA TLSTDTKDRA LRTAADHVLM NTRAILEANE 70 80 90 100 110 120 ADLDTARSSG TPEAMLDRLA LTPARVEGIA DGLRQVAGLP DPVGEVLRGR TLPNGLQLRQ 130 140 150 160 170 180 QRVPLGVVGI VYEGRPNVTV DAFGLTLKSG NAVLLRGSSS AARSNAALVD ALRAALATEL 190 200 210 220 230 240 LDVDAVQLLP SADRASVTHL IQARGLVDVV IPRGGAGLID AVVRDAQVPT IETGVGNCHV 250 260 270 280 290 300 YVHSSADLDT AESIVLNAKT RRPSVCNAAE SLLIDAAVAD VAVPRLTDAL QAAGVTVHID 310 320 330 340 350 360 PTEDELRAEF LSMDIALAIV DGVDGAISHI NEYGTGHTEA IVTTDLAAAQ RFTERVDAAA 370 380 390 400 410 VMVNASTAFT DGEQFGFGAE IGISTQKLHA RGPMGLPELT STKWIVWGDG HTRPA

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP45121.1.
RefSeq	YP_001133909.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T2I0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T2I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000022624; EBMYCP00000022286; EBMYCG00000022619.
GeneID	4973965.
GenomeReviews	Gene locus Mflv_2644 in contig CP000656_GR.
KEGG	mgi:Mflv_2644.
PATRIC	18033437. VBIMycGil17082_2703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
GeneTree	EBGT00050000015663.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	A4T2I0.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_2644-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_MYCGI

Accession

Primary (citable) accession number: A4T2I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program