A4T1T9 (RL2_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	Mflv_5046

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP MF_01320_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	50S ribosomal protein L2 HAMAP MF_01320_B		PRO_1000086338

Sequences

	Sequence		Length	Mass (Da)
	A4T1T9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: B0F68B083970E3EA	FASTA	279	30,515
	10 20 30 40 50 60 MAIRKYKPTT PGRRGSSVSD FAEITRDHPE KSLIRPLHGK GGRNAHGRIT TRHKGGGHKR 70 80 90 100 110 120 AYRVIDFRRH DKDGVDAKVA HIEYDPNRTA NIALLHYLDG EKRYIIAPQG LKQGAIVESG 130 140 150 160 170 180 ANADIKPGNN LPLRNIPAGT LIHAVELRPG GGAKMARSAG ASIQLLGKEG TYASLRMPSG 190 200 210 220 230 240 EIRRVDVRCR ATVGEVGNAE QANINWGKAG RMRWKGKRPT VRGVVMNPVD HPHGGGEGKT 250 260 270 SGGRHPVSPW GKPEGRTRKP NKASDKLIVR RRRTGKNKR

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP47512.1.
RefSeq	YP_001136300.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T1T9.
SMR	A4T1T9. Positions 2-271.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T1T9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000024685; EBMYCP00000024347; EBMYCG00000024680.
GeneID	4976357.
GenomeReviews	Gene locus Mflv_5046 in contig CP000656_GR.
KEGG	mgi:Mflv_5046.
PATRIC	18038358. VBIMycGil17082_5145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0090.
GeneTree	EBGT00050000016403.
HOGENOM	HBG748739.
OMA	QSNINWG.
ProtClustDB	PRK09374.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_5046-MONOMER.
Family and domain databases
HAMAP	MF_01320_B. Ribosomal_L2_B. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR005880. Ribosomal_L2_bac-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
KO	K02886.
PANTHER	PTHR13691:SF5. Ribosom_L2_bac. 1 hit. PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. RplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL2_MYCGI

Accession

Primary (citable) accession number: A4T1T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program