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Protein

Demethylmenaquinone methyltransferase

Gene

menG

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2).UniRule annotation

Catalytic activityi

A demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine.UniRule annotation

Pathwayi: menaquinone biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Demethylmenaquinone methyltransferase (menG)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62S-adenosyl-L-methionineUniRule annotation1
Binding sitei80S-adenosyl-L-methionineUniRule annotation1
Binding sitei117S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00079; UER00169.

Names & Taxonomyi

Protein namesi
Recommended name:
Demethylmenaquinone methyltransferaseUniRule annotation (EC:2.1.1.163UniRule annotation)
Gene namesi
Name:menGUniRule annotation
Ordered Locus Names:Mflv_5258
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000882851 – 228Demethylmenaquinone methyltransferaseAdd BLAST228

Interactioni

Protein-protein interaction databases

STRINGi350054.Mflv_5258.

Structurei

3D structure databases

ProteinModelPortaliA4T183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 101S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249464.
KOiK03183.
OMAiGGLHRAW.
OrthoDBiPOG091H01LK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4T183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRASLEKNP HEVASMFDGV ARRYDLTNTV LSLGQDRFWR RATREALQLS
60 70 80 90 100
PADKVLDLAA GTAVSTVELA SSGAWCVAAD FSVGMLAAGA SRRVPKVAGD
110 120 130 140 150
ATRLPFDDGV FDAVTISFGL RNVVDFSAGL REMARVTRPG GRLVVCEFST
160 170 180 190 200
PTNGLFSTVY KEYLMKALPA MATAVSSNPD AYVYLAESIR AWPDQRALAR
210 220
RIEAAGWSDV RWRNLTGGIV ALHAATKP
Length:228
Mass (Da):24,640
Last modified:May 15, 2007 - v1
Checksum:iF16EAB67E96A538A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP47722.1.
RefSeqiWP_011896078.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP47722; ABP47722; Mflv_5258.
KEGGimgi:Mflv_5258.
PATRICi18038818. VBIMycGil17082_5371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP47722.1.
RefSeqiWP_011896078.1. NC_009338.1.

3D structure databases

ProteinModelPortaliA4T183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_5258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP47722; ABP47722; Mflv_5258.
KEGGimgi:Mflv_5258.
PATRICi18038818. VBIMycGil17082_5371.

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249464.
KOiK03183.
OMAiGGLHRAW.
OrthoDBiPOG091H01LK.

Enzyme and pathway databases

UniPathwayiUPA00079; UER00169.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMENG_MYCGI
AccessioniPrimary (citable) accession number: A4T183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.