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A4T174 (MEND_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:Mflv_5263
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence caution

The sequence ABP47727.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5445442-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341777

Sequences

Sequence LengthMass (Da)Tools
A4T174 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: 30AEC430E340438F

FASTA54456,770
        10         20         30         40         50         60 
MNPSTAQARV VVDELVRGGV RDVVLCPGSR NAPLAFALHD ADRAGRLRLH VRIDERTAGF 

        70         80         90        100        110        120 
LAIGLAVAER APVCVAMTSG TAVANLGPAV VEANYARVPL IVLTANRPYE LLGTGANQTF 

       130        140        150        160        170        180 
EQLGYFGTQV RESISLGLAG DHADMGSLNA QWRSATCRVL VAAKGSRSAN AGPVQFDIPL 

       190        200        210        220        230        240 
REPLVPDADD GSAYAPEGRP GGKEWTYTPA VTFDQPLEID LTPDTVVIAG HGAGVHPNLA 

       250        260        270        280        290        300 
HLPTVAEPTA PYAQTPLHPL ALSLIRPRQV IMLGRPTLHR SVSTLLADPA VPVFALTTGP 

       310        320        330        340        350        360 
RWPDVSGNSQ ATGTRAVTSG AASPEWLRRC AEVNRHAVEA VRGQLAAHPL TTGLHVAAVV 

       370        380        390        400        410        420 
ADAVGPGDQL VLGASNPVRD IALVGFNTAD VKVRSNRGVA GIDGTVSTAI GAALAHERAT 

       430        440        450        460        470        480 
GGRTVALIGD LTFVHDSSGL LVGPTEPTPR KLTIVVSNDN GGGIFELLEQ GDPRFSDVSS 

       490        500        510        520        530        540 
RIFGTPHDVD VGALCRAYHV ESTQIEAGEL AAALAEDFDG MRVLEVKADR SSLRALHASI 


KAGL 

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PYR-GCK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000656 Genomic DNA. Translation: ABP47727.1. Different initiation.
RefSeqYP_001136515.1. NC_009338.1.

3D structure databases

ProteinModelPortalA4T174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350054.Mflv_5263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP47727; ABP47727; Mflv_5263.
GeneID4976572.
KEGGmgi:Mflv_5263.
PATRIC18038828. VBIMycGil17082_5376.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OrthoDBEOG6NWBQW.

Enzyme and pathway databases

BioCycMGIL350054:GHK8-5317-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

Gene3D3.40.50.970. 2 hits.
HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_MYCGI
AccessionPrimary (citable) accession number: A4T174
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways