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A4T174

- MEND_MYCGI

UniProt

A4T174 - MEND_MYCGI

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Protein
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Gene
menD, Mflv_5263
Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity.UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese By similarity.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-5317-MONOMER.
UniPathwayiUPA00079; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (EC:2.2.1.9)
Short name:
SEPHCHC synthase
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene namesi
Name:menD
Ordered Locus Names:Mflv_5263
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5445442-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation
PRO_0000341777Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_5263.

Structurei

3D structure databases

ProteinModelPortaliA4T174.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A4T174-1 [UniParc]FASTAAdd to Basket

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MNPSTAQARV VVDELVRGGV RDVVLCPGSR NAPLAFALHD ADRAGRLRLH    50
VRIDERTAGF LAIGLAVAER APVCVAMTSG TAVANLGPAV VEANYARVPL 100
IVLTANRPYE LLGTGANQTF EQLGYFGTQV RESISLGLAG DHADMGSLNA 150
QWRSATCRVL VAAKGSRSAN AGPVQFDIPL REPLVPDADD GSAYAPEGRP 200
GGKEWTYTPA VTFDQPLEID LTPDTVVIAG HGAGVHPNLA HLPTVAEPTA 250
PYAQTPLHPL ALSLIRPRQV IMLGRPTLHR SVSTLLADPA VPVFALTTGP 300
RWPDVSGNSQ ATGTRAVTSG AASPEWLRRC AEVNRHAVEA VRGQLAAHPL 350
TTGLHVAAVV ADAVGPGDQL VLGASNPVRD IALVGFNTAD VKVRSNRGVA 400
GIDGTVSTAI GAALAHERAT GGRTVALIGD LTFVHDSSGL LVGPTEPTPR 450
KLTIVVSNDN GGGIFELLEQ GDPRFSDVSS RIFGTPHDVD VGALCRAYHV 500
ESTQIEAGEL AAALAEDFDG MRVLEVKADR SSLRALHASI KAGL 544
Length:544
Mass (Da):56,770
Last modified:July 1, 2008 - v2
Checksum:i30AEC430E340438F
GO

Sequence cautioni

The sequence ABP47727.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP47727.1. Different initiation.
RefSeqiYP_001136515.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP47727; ABP47727; Mflv_5263.
GeneIDi4976572.
KEGGimgi:Mflv_5263.
PATRICi18038828. VBIMycGil17082_5376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP47727.1 . Different initiation.
RefSeqi YP_001136515.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4T174.
ModBasei Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_5263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP47727 ; ABP47727 ; Mflv_5263 .
GeneIDi 4976572.
KEGGi mgi:Mflv_5263.
PATRICi 18038828. VBIMycGil17082_5376.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 ; UER00164 .
BioCyci MGIL350054:GHK8-5317-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiMEND_MYCGI
AccessioniPrimary (citable) accession number: A4T174
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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