A4T134 (NHAA1_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Na(+)/H(+) antiporter NhaA 1

Alternative name(s):
Sodium/proton antiporter NhaA 1

Gene names

Name:	nhaA1
Ordered Locus Names:	Mflv_0238

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium gilvum

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Na+/H+ antiporter that extrudes sodium in exchange for external protons By similarity. HAMAP-Rule MF_01844
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01844.
Sequence similarities	In the N-terminal section; belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family. [View classification] Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Antiport Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of pH Inferred from electronic annotation. Source: InterPro sodium ion transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	antiporter activity Inferred from electronic annotation. Source: HAMAP sodium ion transmembrane transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Na(+)/H(+) antiporter NhaA 1 HAMAP-Rule MF_01844		PRO_0000334478
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Transmembrane	198 – 218	21	Helical; Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Transmembrane	304 – 324	21	Helical; Potential
	Transmembrane	341 – 361	21	Helical; Potential
	Transmembrane	378 – 398	21	Helical; Potential
	Transmembrane	411 – 431	21	Helical; Potential
	Domain	434 – 617	184	Thioredoxin
	Region	1 – 433	433	Na(+)/H(+) antiporter NhaA HAMAP-Rule MF_01844

Sequences

	Sequence		Length	Mass (Da)
	A4T134 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 7A1D9B86D9981B39	FASTA	617	66,574
	10 20 30 40 50 60 MTVTEPATQR GFPLLPSRLS RGSKATRTTD NTAAALLLTF TVVAILWANS PWAHTYSALL 70 80 90 100 110 120 DTHVGFSFGS HHAEMTVKHV VNDALMTFFF FIVGLEVTRE FTIGELTDRS RAAVPVVAAA 130 140 150 160 170 180 AGLILPAVVF LAFNPSGENA HAWGVVISTD TAFLVGALAI IKPKFPARVR LFLLTLAVVD 190 200 210 220 230 240 DVGALIAIAV LYSDNIQVAP LVVAVALLGA LALVRYLPKA RGPAYAVLGA ALWIALYLAG 250 260 270 280 290 300 IHPTLAGVAV ALLIPVFTPE RAPVERAVQQ IRAFRQSPNS RYARAASRSL RDSISINERL 310 320 330 340 350 360 QTAVSPVVSF VILPLFALVN AGVLLDGPSL TAALRSPLTW GIVAGLVVGK FVGIAGATWL 370 380 390 400 410 420 IRRTGLGVLA PGLTLRRIAG GAALSGIGFT ISLFIVDIAI DDPSRQDQAR IGVLAASVLA 430 440 450 460 470 480 FALGWAIFRI TDWLSPPEPV GLKLLRPIDP ERDHVRGRPD APLTLVEYGD FECPFCSRVT 490 500 510 520 530 540 GAIDEVRAHF GDDLLYVWRH FPLERAHPRA FDAARASEAA ALQGRFWEMT HELFTHQDDL 550 560 570 580 590 600 EWSDMYRYAV AAGCDIEQFD QDVRVHSSKV LHRVSDDAED ADAMDLNATP TLFVNGKRHR 610 GPWDAASLIR ALEAGRG

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP42733.1.
RefSeq	YP_001131521.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T134.
ModBase	Search...
Protein-protein interaction databases
STRING	350054.Mflv_0238.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP42733; ABP42733; Mflv_0238.
GeneID	4971860.
KEGG	mgi:Mflv_0238.
PATRIC	18028462. VBIMycGil17082_0245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3004.
HOGENOM	HOG000218489.
OMA	FRQSPNS.
ProtClustDB	CLSK901892.
Enzyme and pathway databases
BioCyc	MGIL350054:GHK8-635-MONOMER.
Family and domain databases
Gene3D	1.20.1530.10. 2 hits. 3.40.30.10. 1 hit.
HAMAP	MF_01844. NhaA. Fused.
InterPro	IPR023171. Na/H_antiporter_dom. IPR004670. NhaA. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF06965. Na_H_antiport_1. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR00773. NhaA. 1 hit.
PROSITE	PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NHAA1_MYCGI

Accession

Primary (citable) accession number: A4T134

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program