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Protein

Catalase-peroxidase

Gene

katG

Organism
Polynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei90 – 901Transition state stabilizerUniRule annotation
Active sitei94 – 941Proton acceptorUniRule annotation
Metal bindingi262 – 2621Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciPNEC312153:GH50-2087-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Pnuc_2044
OrganismiPolynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1)
Taxonomic identifieri312153 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
Proteomesi
  • UP000000231 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1212UniRule annotationAdd
BLAST
Chaini13 – 717705Catalase-peroxidasePRO_0000354860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki93 ↔ 221Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-247)UniRule annotation
Cross-linki221 ↔ 247Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-93)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiA4T0J1.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi312153.Pnuc_2044.

Structurei

3D structure databases

ProteinModelPortaliA4T0J1.
SMRiA4T0J1. Positions 24-717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4T0J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKGMCPVA HGANTEASET PMAWWPKALN LDILHQQDTK TNPMGSSFSY
60 70 80 90 100
RDELKKLDVG ALKKDMKDLL TNSQDWWPAD WGHYGGLMIR MAWHSAGSYR
110 120 130 140 150
IADGRGGAGT GNQRFAPINS WPDNANLDKA RRLLWPIKKK YGNKISWADL
160 170 180 190 200
MILAGTIAYE SMGLKTFGFS FGREDIWHPE KDIYWGSEKE WLQKSGGKGS
210 220 230 240 250
RYSGERELSN PLAAVMMGLI YVNPEGVDGK PDPLKTAQDM RVTFARMAMN
260 270 280 290 300
DEETVALTAG GHTVGKAHGN GNAANLGPAP EAAPIDEQGL GWMNHKTRGI
310 320 330 340 350
GRDAVTSGLE GAWTTHPTQW DNGYFNLLLN YDWKLTESPA GAHQYEPINI
360 370 380 390 400
KEEDKPVDVE DASIRCMPMM TDADIALKMD PEYRKISERF SKDQAYFSET
410 420 430 440 450
FAKAWFKLTH RDMGPKARYF GPDVPKEELI WQDPIPSGPK SYDIDAVKAK
460 470 480 490 500
IKASGLSMSD MVTTAWDSAR TFRGSDKRGG ANGARIRLAP QKDWMGNEPE
510 520 530 540 550
RLARVLAVYE KIGKECGISI ADTIILGGNI GIEQAAKAGG FDVKVPFTSG
560 570 580 590 600
RGDAIQAMTD VESFEVLEPL ADGFRNWLKE SYVVTPEELL LDRTQLMGLT
610 620 630 640 650
AQEMTVLIGG MRVLGTNYGG SKQGVFTEKE GVLSNDFFVN LTDMNYLWKP
660 670 680 690 700
TGQNSYDIVE RNTEKTKWTA TRADLVFGSN SILRAYAEVY AQDDNKEKFV
710
NDFIAAWTKV MNADLFN
Length:717
Mass (Da):79,677
Last modified:May 15, 2007 - v1
Checksum:i123162FCD1A2A41B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000655 Genomic DNA. Translation: ABP35255.1.
RefSeqiWP_011903878.1. NC_009379.1.

Genome annotation databases

EnsemblBacteriaiABP35255; ABP35255; Pnuc_2044.
KEGGipnu:Pnuc_2044.
PATRICi22968619. VBIPolNec12025_2113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000655 Genomic DNA. Translation: ABP35255.1.
RefSeqiWP_011903878.1. NC_009379.1.

3D structure databases

ProteinModelPortaliA4T0J1.
SMRiA4T0J1. Positions 24-717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312153.Pnuc_2044.

Proteomic databases

PRIDEiA4T0J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP35255; ABP35255; Pnuc_2044.
KEGGipnu:Pnuc_2044.
PATRICi22968619. VBIPolNec12025_2113.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Enzyme and pathway databases

BioCyciPNEC312153:GH50-2087-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_POLSQ
AccessioniPrimary (citable) accession number: A4T0J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.