A4T050 (A4T050_POLSQ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817
EC=1.7.1.13 HAMAP MF_00817

Alternative name(s):
7-cyano-7-carbaguanine reductase HAMAP MF_00817
NADPH-dependent nitrile oxidoreductase HAMAP MF_00817
PreQ(0) reductase HAMAP MF_00817

Gene names

Name:	queF HAMAP MF_00817
Ordered Locus Names:	Pnuc_1902

Organism

Polynucleobacter sp. (strain QLW-P1DMWA-1) [Complete proteome] [HAMAP] EMBL ABP35114.1

Taxonomic identifier

312153 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. HAMAP MF_00817

Ontologies

Keywords
Biological process	Queuosine biosynthesis HAMAP MF_00817
Cellular component	Cytoplasm HAMAP MF_00817
Ligand	NADP HAMAP MF_00817
Molecular function	Hydrolase EMBL ABP35114.1 Oxidoreductase HAMAP MF_00817
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A4T050 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 7D579D181D59D5E0	FASTA	275	31,070
	10 20 30 40 50 60 MATLPLGQST QYPDQYDPSL LFPIPRTENR AKLGLKEGQA LPFVGIDIWN AFELSWLNQK 70 80 90 100 110 120 GKPQIALAEF QIPADSPNMI ESKSFKLYLN SLNSARFEDE GQVKDRLITD LSAVAGSKVA 130 140 150 160 170 180 TRIHSTEAVS KKGMQEMSGI LMDRLDIEID PHLPADPSLL GVNESFGPIE QCLVSHLLKS 190 200 210 220 230 240 NCPVTGQPDW ASVQIRYQGR PILEEGLLRY LIGFRQLGEF HEHCVETIFT DIKRQCKPEK 250 260 270 LSVYARYTRR GGLDINPFRT DHNSPWPENT RHARQ

References

[1]

"Complete sequence of Polynucleobacter sp. QLW-P1DMWA-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Poeckl M., Wu Q.L., Hahn M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QLW-P1DMWA-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000655 Genomic DNA. Translation: ABP35114.1.
RefSeq	YP_001156678.1. NC_009379.1.
3D structure databases
ProteinModelPortal	A4T050.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T050.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5053718.
GenomeReviews	Gene locus Pnuc_1902 in contig CP000655_GR.
KEGG	pnu:Pnuc_1902.
PATRIC	22968327. VBIPolNec12025_1968.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
PhylomeDB	A4T050.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	PSP312153:PNUC_1902-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4T050_POLSQ

Accession

Primary (citable) accession number: A4T050

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)