A4SZM1 (SYQ_POLSQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Pnuc_1722

Organism

Polynucleobacter sp. (strain QLW-P1DMWA-1) [Complete proteome] [HAMAP]

Taxonomic identifier

312153 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Glutamine--tRNA ligase HAMAP MF_00126		PRO_1000095497
Regions
	Motif	55 – 65	11	"HIGH" region HAMAP MF_00126
	Motif	299 – 303	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	302	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SZM1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: DDEDA6ADF43DB4E2	FASTA	590	67,427
	10 20 30 40 50 60 MSQDSKPTKS AAGAIAEPSN FLRQIIDHDL ASGAFAQRTN LAGEAIPSII TRFPPEPNGY 70 80 90 100 110 120 LHIGHAKSIC LNFGLAADYN NQSGGARCNM RLDDTNPVKE DVEYADSILD AVKWLGFDWG 130 140 150 160 170 180 THLYHASDYF DRLYEFAEIL IQNSKAYVDS QSADDIHTNR GNFGQVGKNS PYRERTPDEN 190 200 210 220 230 240 LQLFREMRDG KFKDGEHVLR LKIDMAHPNI VMRDPVVYRI RHTDHHRTGS KWCIYPLYDF 250 260 270 280 290 300 THCISDALEN VSHSICTLEF ENNRPLYDWI VNSLKELGVF KDPVPHQYEF ARLNLTYTIT 310 320 330 340 350 360 SKRKLLQLVE EQHVEGWDDP RMPTIVGIRR RGYTPESIRL FCERIGVSKA DSWIDMSTLD 370 380 390 400 410 420 QALRDDLEAR APRATAVLKP LKLVVENFDA AHSEACSAPR HPHHPEWGNR EFNFTKELWI 430 440 450 460 470 480 EADDFMQEPV KGFFRLYPPI GDQPGSRVRL RHGFVVECTD FETDAKGNIT QVNVNYFPDS 490 500 510 520 530 540 KSGTPGSNNY KVKGNIHWIS AAEAIPAEVR LYDHLFTDPH PDSGDKNFLD TINPHSKETI 550 560 570 580 590 KAYLEPCMKD VKPEDRFQFE RHGYFVADQN DSAPGKPVFN RTVGLKDSWK

References

[1]

"Complete sequence of Polynucleobacter sp. QLW-P1DMWA-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Poeckl M., Wu Q.L., Hahn M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QLW-P1DMWA-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000655 Genomic DNA. Translation: ABP34935.1.
RefSeq	YP_001156499.1. NC_009379.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SZM1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5052005.
GenomeReviews	Gene locus Pnuc_1722 in contig CP000655_GR.
KEGG	pnu:Pnuc_1722.
PATRIC	22967965. VBIPolNec12025_1788.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
PhylomeDB	A4SZM1.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	PSP312153:PNUC_1722-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_POLSQ

Accession

Primary (citable) accession number: A4SZM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program