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A4SZM1 (SYQ_POLSQ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:Pnuc_1722
OrganismPolynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1) [Complete proteome] [HAMAP]
Taxonomic identifier312153 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095497

Regions

Motif55 – 6511"HIGH" region HAMAP-Rule MF_00126
Motif299 – 3035"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site3021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SZM1 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: DDEDA6ADF43DB4E2

FASTA59067,427
        10         20         30         40         50         60 
MSQDSKPTKS AAGAIAEPSN FLRQIIDHDL ASGAFAQRTN LAGEAIPSII TRFPPEPNGY 

        70         80         90        100        110        120 
LHIGHAKSIC LNFGLAADYN NQSGGARCNM RLDDTNPVKE DVEYADSILD AVKWLGFDWG 

       130        140        150        160        170        180 
THLYHASDYF DRLYEFAEIL IQNSKAYVDS QSADDIHTNR GNFGQVGKNS PYRERTPDEN 

       190        200        210        220        230        240 
LQLFREMRDG KFKDGEHVLR LKIDMAHPNI VMRDPVVYRI RHTDHHRTGS KWCIYPLYDF 

       250        260        270        280        290        300 
THCISDALEN VSHSICTLEF ENNRPLYDWI VNSLKELGVF KDPVPHQYEF ARLNLTYTIT 

       310        320        330        340        350        360 
SKRKLLQLVE EQHVEGWDDP RMPTIVGIRR RGYTPESIRL FCERIGVSKA DSWIDMSTLD 

       370        380        390        400        410        420 
QALRDDLEAR APRATAVLKP LKLVVENFDA AHSEACSAPR HPHHPEWGNR EFNFTKELWI 

       430        440        450        460        470        480 
EADDFMQEPV KGFFRLYPPI GDQPGSRVRL RHGFVVECTD FETDAKGNIT QVNVNYFPDS 

       490        500        510        520        530        540 
KSGTPGSNNY KVKGNIHWIS AAEAIPAEVR LYDHLFTDPH PDSGDKNFLD TINPHSKETI 

       550        560        570        580        590 
KAYLEPCMKD VKPEDRFQFE RHGYFVADQN DSAPGKPVFN RTVGLKDSWK 

« Hide

References

[1]"Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1(T))."
Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M., Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.
Stand. Genomic Sci. 6:74-83(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 18221 / CIP 109841 / QLW-P1DMWA-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000655 Genomic DNA. Translation: ABP34935.1.
RefSeqYP_001156499.1. NC_009379.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING312153.Pnuc_1722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP34935; ABP34935; Pnuc_1722.
GeneID5052005.
KEGGpnu:Pnuc_1722.
PATRIC22967965. VBIPolNec12025_1788.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycPNEC312153:GH50-1762-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_POLSQ
AccessionPrimary (citable) accession number: A4SZM1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 15, 2007
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries