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A4SWW0 (MDH_POLSQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase
EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Pnuc_0756
OrganismPolynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1) [Complete proteome] [HAMAP]
Taxonomic identifier312153 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter ›

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Malate dehydrogenase HAMAP-Rule MF_01517
PRO_1000087544

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding132 – 1343NAD By similarity

Sites

Active site1901Proton acceptor By similarity
Binding site951Substrate By similarity
Binding site1011Substrate By similarity
Binding site1081NAD By similarity
Binding site1151NAD By similarity
Binding site1341Substrate By similarity
Binding site1651Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SWW0 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 487D813091C5CC0A

FASTA32935,502
        10         20         30         40         50         60 
MAKAPMRVAV TGAAGQIGYS LLFRIANGDL LGKDQPVILQ LLEIPDEKAQ KALTGVMMEL 

        70         80         90        100        110        120 
EDCAFPLLAG MTAHSDPMTA FKDIDVALLV GARPRGPGME RKDLLSANAQ IFTAQGKALN 

       130        140        150        160        170        180 
AVAKKTVKVL VVGNPANTNA YIAMKSAPDI PAKNFTAMLR LDHNRALSQL ANKLNKPVAD 

       190        200        210        220        230        240 
IEKLVVWGNH SPTMYPDYRF ATIDGKSVKD SINDAAWNKD VFIPTVGKRG AAIIEARGLS 

       250        260        270        280        290        300 
SAASAANAAI DHIHDWVLGT NGKWVTMGIP SKGEYGIPAE VIYGFPVTCE NGEYKMIEGL 

       310        320 
EIDEFSRERM THTLNELLEE QAGVKHLLP

« Hide

References

[1]"Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1(T))."
Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M., Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.
Stand. Genomic Sci. 6:74-83(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 18221 / CIP 109841 / QLW-P1DMWA-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000655 Genomic DNA. Translation: ABP33974.1.
RefSeqYP_001155538.1. NC_009379.1.

3D structure databases

ProteinModelPortalA4SWW0.
SMRA4SWW0. Positions 3-328.
ModBaseSearch...

Protein-protein interaction databases

STRING312153.Pnuc_0756.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP33974; ABP33974; Pnuc_0756.
GeneID5053149.
KEGGpnu:Pnuc_0756.
PATRIC22965919. VBIPolNec12025_0786.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000220953.
KOK00024.
OMANCLIASK.
ProtClustDBPRK05442.

Enzyme and pathway databases

BioCycPNEC312153:GH50-800-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_POLSQ
AccessionPrimary (citable) accession number: A4SWW0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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