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A4SVD9 (SYL_POLSQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pnuc_0232
OrganismPolynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1) [Complete proteome] [HAMAP]
Taxonomic identifier312153 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074838

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif645 – 6495"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SVD9 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: B9ED38A7A3BF2954

FASTA89099,554
        10         20         30         40         50         60 
MSKDYDHRGI EAAAQADWER TQVYRVTENA VNASGKKKPK YYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDVMAR QLRMQGYNVL MPMGWDAFGM PAENAAIQNK VPPAKWTYDN IAYMKKQMAA 

       130        140        150        160        170        180 
MGLAIDWSRE VATCSPDYYR WNQWLFLKML EKGVAYRKTQ VVNWDPIDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGALVE KREIPGYYFN ITAYAEQLLS GLDGLGWPER VKTMQQNWIG KSRGVRFAFK 

       250        260        270        280        290        300 
HEIADAHGNF IQDGLLYVFT TRADTIMGVT FCAVAAEHPL ASKAAENNPT LAAFIEKCKT 

       310        320        330        340        350        360 
GSVIEADLAT QEKEGMFTGL YVTHPLTNES VPVWVGNYVL MSYGDGAVMG VPAHDERDFA 

       370        380        390        400        410        420 
FALKYELPIK QVIALKDESP MFNGTHWQDW YAQKENVVCF NSGKFDGLSH EEAVNTVASD 

       430        440        450        460        470        480 
LEKMGIGELK TTYRLRDWGI SRQRYWGTPI PIIHCGDEGN PGCGAVPVPE ADLPVVLPED 

       490        500        510        520        530        540 
CVPDGSGNPL NKRADFLNVK CPQCGKPARR ETDTMDTFVD SSWYFMRYTG PDATSMVDGR 

       550        560        570        580        590        600 
NEYWMPMDQY IGGIEHAILH LLYARFWTKV MRDLDLITFD EPFQNLLTQG MVLNETYYSE 

       610        620        630        640        650        660 
EASGKKTWLN PLDVELDLDE KGRPQGAKLK GDSSGTPVII GGVEKMSKSK NNGVDPQALI 

       670        680        690        700        710        720 
DQYGADTARL FVMFAAPPEQ QLEWSGAGVD GASRFLRRVW MYSSSQASAI KDAQDALPST 

       730        740        750        760        770        780 
LNDAEKELRR EVHAILKQAN FDYQRRQYNT VVSAAMKMLN VLEPIKLGQD SPISASVLRE 

       790        800        810        820        830        840 
CLSILLRVLY PVVPHLTHVL WKDMGYSENF GSLLDAPWPT VDETALIQTE ITLMLQINGK 

       850        860        870        880        890 
LRGELKVPAD ASKEQIEAIA LQSEPATKAL NGGAPKKVIV VPGRLINIVA 

« Hide

References

[1]"Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1(T))."
Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M., Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.
Stand. Genomic Sci. 6:74-83(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 18221 / CIP 109841 / QLW-P1DMWA-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000655 Genomic DNA. Translation: ABP33453.1.
RefSeqYP_001155017.1. NC_009379.1.

3D structure databases

ProteinModelPortalA4SVD9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING312153.Pnuc_0232.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP33453; ABP33453; Pnuc_0232.
GeneID5053383.
KEGGpnu:Pnuc_0232.
PATRIC22964813. VBIPolNec12025_0239.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPNEC312153:GH50-243-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_POLSQ
AccessionPrimary (citable) accession number: A4SVD9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries