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A4SV63 (BIOB_POLSQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Biotin synthase HAMAP-Rule MF_01694
PRO_0000381531

Sites

Metal binding761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1201Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1511Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2111Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2831Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SV63 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 559DE052C44FE3F3

FASTA34537,446
        10         20         30         40         50         60 
MLDAQTVEKP LTHIKSKADL RKEVDASGEW TVSQIEALFA LPFNELMFKA QQAHEANFPE 

        70         80         90        100        110        120 
GDVELATLLS IKTGGCPEDC SYCPQAARYD TDVKAEKLMG LEEVLEAAKA AKAAGSNRFC 

       130        140        150        160        170        180 
MGAAWREPKD RDIEKVTAMI KGVKALGLET CATLGMLEAN QAKALQEAGL DFYNHNLDTS 

       190        200        210        220        230        240 
EDFYRSVIST RGYQDRLDTI SNVRAAGMSV CCGGIVGMGE SREQRAAFLA RLANLSPYPE 

       250        260        270        280        290        300 
SVPINHLVPV AGTPLAEQKP LDPLEFVRTI AVARITMPKA RVRLSAGRQE LGRAIQAMCF 

       310        320        330        340 
MAGANSIFYG EQLLTTGNPE AEQDLALLAE LGLKTKQSCK AEVLV 

« Hide

References

[1]"Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1(T))."
Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M., Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.
Stand. Genomic Sci. 6:74-83(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 18221 / CIP 109841 / QLW-P1DMWA-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000655 Genomic DNA. Translation: ABP33377.1.
RefSeqYP_001154941.1. NC_009379.1.

3D structure databases

ProteinModelPortalA4SV63.
SMRA4SV63. Positions 29-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING312153.Pnuc_0156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP33377; ABP33377; Pnuc_0156.
GeneID5053152.
KEGGpnu:Pnuc_0156.
PATRIC22964659. VBIPolNec12025_0162.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPNEC312153:GH50-167-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_POLSQ
AccessionPrimary (citable) accession number: A4SV63
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways