A4STF3 (FADA_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-ketoacyl-CoA thiolase
EC=2.3.1.16

Alternative name(s):
Fatty acid oxidation complex subunit beta
Beta-ketothiolase
Acetyl-CoA acyltransferase

Gene names

Name:	fadA
Ordered Locus Names:	ASA_4251

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed By similarity. HAMAP MF_01620
Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA. HAMAP MF_01620
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01620
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01620.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	3-ketoacyl-CoA thiolase HAMAP MF_01620		PRO_0000292885
Sites
	Active site	91	1	Acyl-thioester intermediate By similarity
	Active site	343	1	Proton acceptor By similarity
	Active site	373	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4STF3-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 8B2FDB2583A0695D	FASTA	387	40,976
	10 20 30 40 50 60 MKDVVIVDCI RTPMGRSKGG AFRNVRAEDL SAHLMSSILL RNPNLDPNEI EDIYWGCVQQ 70 80 90 100 110 120 TLEQGFNIAR NAALLAGIPK QVGAVTVNRL CGSSMQALHD ASRAIQVGDG DIFIIGGVEH 130 140 150 160 170 180 MGHVPMSHGV DFHPGMAKSV AKASGMMGLT AEMLGKLHGI SRQQQDEFAA RSHRRAYAAT 190 200 210 220 230 240 VEGRFAREIV GLEGHDASGA RFFYDYDEVI RPETTVETLS QLRPVFDPVN GTVTAGTSSA 250 260 270 280 290 300 LSDGASAMLV MSADRAKALG LTPRVRVRSM AVAGCDAAIM GYGPVPATQK ALKRAGLTIG 310 320 330 340 350 360 DIDLVELNEA FAAQSLPCVK DLGLLDVAEE KVNLNGGAIA LGHPLGCSGS RISTTLIHLM 370 380 EEKDANLGLA TMCIGLGQGI ATVFERV

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO92175.1.
RefSeq	YP_001143923.1.
3D structure databases
ProteinModelPortal	A4STF3.
SMR	A4STF3. Positions 2-387.
ModBase	Search...
Protein-protein interaction databases
STRING	A4STF3.
Genome annotation databases
GeneID	4998358.
GenomeReviews	Gene locus ASA_4251 in contig CP000644_GR.
KEGG	asa:ASA_4251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	AIDDIYW.
PhylomeDB	A4STF3.
ProtClustDB	PRK08947.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_4251-MONOMER.
Family and domain databases
HAMAP	MF_01620. FadA. [Tree]
InterPro	IPR012805. FadA. IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit. TIGR02445. fadA. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADA_AERS4

Accession

Primary (citable) accession number: A4STF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	May 15, 2007
Last modified:	August 10, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)