Reviewed, UniProtKB/Swiss-Prot A4STF2 (FADB_AERS4)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.3.3.8
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadB
Ordered Locus Names:	ASA_4250

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01621 (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01621 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01621 (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. HAMAP MF_01621
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01621
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: HAMAP enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Fatty acid oxidation complex subunit alpha HAMAP MF_01621		PRO_1000069558
Regions
	Region	1 – 189	189	Enoyl-CoA hydratase/isomerase By similarity
	Region	312 – 715	404	3-hydroxyacyl-CoA dehydrogenase By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4STF2-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 0B02B7D7F59A55EF	FASTA	715	76,376
	10 20 30 40 50 60 MIYQGETLSV SYLENGIAEL RFDAPGSVNK LDRATLLSLS EAIAALQQQA DLKGLILTSG 70 80 90 100 110 120 KDAFIVGADI TEFLELFDLP QEDLLGWLKK ANDIFSAIED LPVPTLSAIK GHALGGGCET 130 140 150 160 170 180 ILSTDFRLAD TSAKIGLPET KLGIMPGFGG TVRLPRVIGA DNALEWITTG KDYRADDALK 190 200 210 220 230 240 VGAIDAVVAP DALHSAAVQM MKDAIAGKLN WQSRRAAKKA PLRLSKLEAM MSFSTAAGMV 250 260 270 280 290 300 AAVAGKHYPA PMTAVKTVEA AAGMSRDEAL VVEAQGFIKL AKTDVAKALV GIFLNDQHIK 310 320 330 340 350 360 ALAKKAAKQA AKATRHAAVL GAGIMGGGIA YQSASKGIPA VMKDINEKAL ALGMGEATKL 370 380 390 400 410 420 LNGQLEKGRI DGIKMGQVLS AITPTLSYDN VKHVDLVVEA VVENPKVKAA VLGEVEGIIG 430 440 450 460 470 480 DDAVLASNTS TIPISLLAKG LKRPQNFCGM HFFNPVHRMP LVEIIRGEQT SDETINRVVA 490 500 510 520 530 540 YAAAMGKSPV VVNDCPGFFV NRVLFPYFFG FNKLVADGAD FAAVDKVMEK EFGWPMGPAY 550 560 570 580 590 600 LLDVVGIDTG HHAGDVMAQG FPARMSKEGR TAIDVMYDAS RFGQKNGKGF YAYEQDKKGK 610 620 630 640 650 660 PKKVADVAAY ELLAPIAKPK QDFDKEAIIA GMMIPMINEV VLCLEEGIVA TPAEADIALV 670 680 690 700 710 YGLGFPPFRG GVFRYLDTIG LDRYVAMADQ YADLGPLYRV SDRLREMAAQ GKTFY

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO92174.1.
RefSeq	YP_001143922.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4998357.
GenomeReviews	Gene locus ASA_4250 in contig CP000644_GR.
KEGG	asa:ASA_4250.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4STF2. ANNGSYY.
Family and domain databases
HAMAP	MF_01621. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012799. FadB. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00725. 3HCDH. 2 hits. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02437. FadB. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADB_AERS4

Accession

Primary (citable) accession number: A4STF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 15, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)