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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei325NAD; via amide nitrogenUniRule annotation1
Binding sitei344NADUniRule annotation1
Binding sitei408NADUniRule annotation1
Binding sitei430NADUniRule annotation1
Active sitei451For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei454NADUniRule annotation1
Binding sitei501SubstrateUniRule annotation1
Binding sitei661SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi401 – 403NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:ASA_4250
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000695581 – 715Fatty acid oxidation complex subunit alphaAdd BLAST715

Proteomic databases

PRIDEiA4STF2

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi382245.ASA_4250

Structurei

3D structure databases

ProteinModelPortaliA4STF2
SMRiA4STF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7153-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST405

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiIDLCMIL

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A4STF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQGETLSV SYLENGIAEL RFDAPGSVNK LDRATLLSLS EAIAALQQQA
60 70 80 90 100
DLKGLILTSG KDAFIVGADI TEFLELFDLP QEDLLGWLKK ANDIFSAIED
110 120 130 140 150
LPVPTLSAIK GHALGGGCET ILSTDFRLAD TSAKIGLPET KLGIMPGFGG
160 170 180 190 200
TVRLPRVIGA DNALEWITTG KDYRADDALK VGAIDAVVAP DALHSAAVQM
210 220 230 240 250
MKDAIAGKLN WQSRRAAKKA PLRLSKLEAM MSFSTAAGMV AAVAGKHYPA
260 270 280 290 300
PMTAVKTVEA AAGMSRDEAL VVEAQGFIKL AKTDVAKALV GIFLNDQHIK
310 320 330 340 350
ALAKKAAKQA AKATRHAAVL GAGIMGGGIA YQSASKGIPA VMKDINEKAL
360 370 380 390 400
ALGMGEATKL LNGQLEKGRI DGIKMGQVLS AITPTLSYDN VKHVDLVVEA
410 420 430 440 450
VVENPKVKAA VLGEVEGIIG DDAVLASNTS TIPISLLAKG LKRPQNFCGM
460 470 480 490 500
HFFNPVHRMP LVEIIRGEQT SDETINRVVA YAAAMGKSPV VVNDCPGFFV
510 520 530 540 550
NRVLFPYFFG FNKLVADGAD FAAVDKVMEK EFGWPMGPAY LLDVVGIDTG
560 570 580 590 600
HHAGDVMAQG FPARMSKEGR TAIDVMYDAS RFGQKNGKGF YAYEQDKKGK
610 620 630 640 650
PKKVADVAAY ELLAPIAKPK QDFDKEAIIA GMMIPMINEV VLCLEEGIVA
660 670 680 690 700
TPAEADIALV YGLGFPPFRG GVFRYLDTIG LDRYVAMADQ YADLGPLYRV
710
SDRLREMAAQ GKTFY
Length:715
Mass (Da):76,376
Last modified:May 15, 2007 - v1
Checksum:i0B02B7D7F59A55EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA Translation: ABO92174.1
RefSeqiWP_005320561.1, NC_009348.1

Genome annotation databases

EnsemblBacteriaiABO92174; ABO92174; ASA_4250
GeneIDi4998357
KEGGiasa:ASA_4250
PATRICifig|382245.13.peg.4215

Similar proteinsi

Entry informationi

Entry nameiFADB_AERS4
AccessioniPrimary (citable) accession number: A4STF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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