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A4STE2 (ILVC_AERS4) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86
Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase
Gene names
Name:ilvC
Ordered Locus Names:ASA_4236
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435

(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Ketol-acid reductoisomerase HAMAP MF_00435
PRO_1000050475

Sites

Active site1321 Potential

Sequences

Sequence LengthMass (Da)Tools
A4STE2 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 917D74D797BB0CF6

FASTA49353,841
        10         20         30         40         50         60 
MANYFNTLNL RQQLAQLGKC RFMQRAEFAD GCDVLKGKKV VIVGCGAQGL NQGLNMRDSG 

        70         80         90        100        110        120 
LDISYTLRKA AITEKRASWQ KATDNGFAVG TYEELIPTAD LVLNLTPDKQ HSDVVKTVMP 

       130        140        150        160        170        180 
LMKQGAALGY SHGFNVVEEG QQIRADITVV MVAPKCPGTE VREEYKRGFG VPTLLAVHPE 

       190        200        210        220        230        240 
NDPKGVGMAI AKAWASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCYDKL 

       250        260        270        280        290        300 
VAEGTDPAYA GKLIQFGWET ITEALKQGGI SLMMDRLSNP AKLRAFELSE QLRTLMRPLF 

       310        320        330        340        350        360 
EKHMDDIIAG EFSRGMMADW AEDDAKLFGW REETGKSAFE NALAFAGKIA EQEYFDNGVV 

       370        380        390        400        410        420 
MVAMVKAGVE LAFETMVASG IYEESAYYES LHELPLIANT VARKRLYEMN VVISDTAEYG 

       430        440        450        460        470        480 
NYLFANAAVP LLREHFMPTL KAGDLGASKA EGQNVDNLAL LAANEATRNH PIEKIGQVLR 

       490 
GYMKDMKRIA VGG

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References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000644 Genomic DNA. Translation: ABO92164.1.
RefSeqYP_001143912.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4STE2.
SMRA4STE2. Positions 3-491.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4STE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4996960.
GenomeReviewsGene locus ASA_4236 in contig CP000644_GR.
KEGGasa:ASA_4236.
PATRIC20794932. VBIAerSal2987_4204.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0059.
HOGENOMHBG297205.
OMAEKHMDDI.
PhylomeDBA4STE2.
ProtClustDBPRK05225.

Enzyme and pathway databases

BioCycASAL382245:ASA_4236-MONOMER.

Family and domain databases

HAMAPMF_00435. IlvC.
[Tree]
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013023. AcH_isomrdctse.
IPR000506. AcH_isomrdctse_C.
IPR013328. DH_multihelical.
IPR013116. IlvN.
IPR014359. KetolA_reductoisomerase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KOK00053.
PANTHERPTHR21371. AcH_isomrdctse. 1 hit.
PTHR21371:SF2. PTHR21371:SF2. 1 hit.
PfamPF01450. IlvC. 2 hits.
PF07991. IlvN. 1 hit.
[Graphical view]
PIRSFPIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAMSSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMsTIGR00465. IlvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameILVC_AERS4
AccessionPrimary (citable) accession number: A4STE2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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