Reviewed, UniProtKB/Swiss-Prot A4STB3 (LEXA_AERS4)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	ASA_4206

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	LexA repressor HAMAP MF_00015		PRO_1000001253
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	124	1	For autocatalytic cleavage activity By similarity
	Active site	161	1	For autocatalytic cleavage activity By similarity
	Site	89 – 90	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4STB3-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E484BE4A4ACE9529	FASTA	207	22,852
	10 20 30 40 50 60 MKPLTPRQAE VLELIKVNMS ETGMPPTRAE IAQKLGFKSA NAAEEHLKAL AKKGVIEIMP 70 80 90 100 110 120 GTSRGIRLLI EEEAVLEETG LPLIGKVAAG EPILAQEHIE SHYQVDPALF HPRADFLLRV 130 140 150 160 170 180 QGMSMKNIGI LDGDLLAVHK TQEVRNGQVV VARLDEDVTV KRFQRKGSQV WLLPENEELE 190 200 PIAVDLSCQQ LTIEGLAVGV IRNADWM

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO92135.1.
RefSeq	YP_001143883.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4STB3.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	4996261.
GenomeReviews	Gene locus ASA_4206 in contig CP000644_GR.
KEGG	asa:ASA_4206.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KVIGVFR.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_AERS4

Accession

Primary (citable) accession number: A4STB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)