Reviewed, UniProtKB/Swiss-Prot A4SSN5 (HUTI_AERS4)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	ASA_3955

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Imidazolonepropionase HAMAP MF_00372		PRO_0000306423
Sites
	Metal binding	75	1	Zinc or iron By similarity
	Metal binding	77	1	Zinc or iron By similarity
	Metal binding	245	1	Zinc or iron By similarity
	Metal binding	320	1	Zinc or iron By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SSN5-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 821EDD22C8AA6D1F	FASTA	411	44,527
	10 20 30 40 50 60 MNKELLNCER VWLNVTPATL RPDLADYGLL EPHALGVHEG KIHALVPMQD LKGPYPAHWQ 70 80 90 100 110 120 DMKGKLATPG LIDCHTHLIF AGSRAEEFEL RQKGVPYAEI ARKGGGILST VRATRAASEE 130 140 150 160 170 180 QLFTLAAARI KSLIREGVTT VEIKSGYGLT LEDELKMLRV ARRLGEALPI RVKTTLLAAH 190 200 210 220 230 240 AVPPEYRDDP DSWVETICQE IIPAAAEAGL ADAVDVFCEH IGFSLAQTEQ VYLAADQYGL 250 260 270 280 290 300 PVKGHMEQLS NLGGSALAAN FGALSVDHLE HLDQEGIQAL AHRGVVATLL PTAFYFLKET 310 320 330 340 350 360 KLPPVAALRK AHVPMAVSSD INPGTAPIVS LRMAMNMACT LFGLTPVEAM VGVTRNAARA 370 380 390 400 410 LGEQERLGQL RVGMLADFLV WNCAHPAELS YLIGVDQLVS RVVNGEETLH G

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO91907.1.
RefSeq	YP_001143655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SSN5.
Genome annotation databases
GeneID	4996312.
GenomeReviews	Gene locus ASA_3955 in contig CP000644_GR.
KEGG	asa:ASA_3955.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MNMACTL.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_AERS4

Accession

Primary (citable) accession number: A4SSN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)