Reviewed, UniProtKB/Swiss-Prot A4SSA7 (AMPA_AERS4)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	ASA_3823

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019876
Sites
	Active site	279	1	Potential
	Active site	353	1	Potential
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	272	1	Manganese 1 By similarity
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	290	1	Manganese 2 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SSA7-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 307109404ED4D70C	FASTA	502	54,676
	10 20 30 40 50 60 MEFSVKSGSP EKQRSACVVV GVFEPRRLSP VAEQLDKISD GYISSLLRRG DLEGKPGQML 70 80 90 100 110 120 LLHQVPGVLS ERVLLVGCGK ERELDERQYK QIINKTITTL NETGSMEAVC FLTELHVKGR 130 140 150 160 170 180 DTYWKVRQAV ETTKAGLYNF DQFKTNKAEP RRPLRKLVFN VPTRRELTIG EKAIAHGLAV 190 200 210 220 230 240 AKGVRVCRDV ANMPPNVCNP AYLASQARRL ADSFDNITTK VIGEQEMAEL GMNSYLAVAR 250 260 270 280 290 300 GSENEAMMAI IEYKGNADAK PIVLVGKGLT FDSGGISIKP AEGMDEMKYD MGGAASVLGT 310 320 330 340 350 360 MHALAQLQLP INVIGVLAGC ENMPGGNAYR PGDILTSMSG QTIEVLNTDA EGRLVLCDAL 370 380 390 400 410 420 TYVDRYDPET VIDVATLTGA CIIALGHHTT GLLANHNPLA HELLNASEQA GDRAWRLPLF 430 440 450 460 470 480 DEYQEQLESP FADMANIGGR PAGTITAAAF LSRFTKKYNW AHLDIAGTAW KSGKDKGSTG 490 500 RPVPLLTQFL LNRAGVDIEE KE

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91779.1.
RefSeq	YP_001143527.1.
3D structure databases
SMR	A4SSA7. Positions 1-500.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SSA7.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	4997692.
GenomeReviews	Gene locus ASA_3823 in contig CP000644_GR.
KEGG	asa:ASA_3823.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	LGHHISG.
PhylomeDB	A4SSA7.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AERS4

Accession

Primary (citable) accession number: A4SSA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	January 19, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)