Reviewed, UniProtKB/Swiss-Prot A4SS99 (SYA_AERS4)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ASA_3811

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347481
Regions
	Zinc finger	177 – 190	14	C2H2-type HAMAP MF_00036

Sequences

	Sequence		Length	Mass (Da)
	A4SS99-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: D25D68EE224BA3F1	FASTA	874	95,779
	10 20 30 40 50 60 MYMSTSEIRA AFLEYFRSQG HQVVSSSSLV PHNDPTLLFT NAGMNQFKDV FLGADKRAYN 70 80 90 100 110 120 RATTSQRCVR AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKQDAIRF AWEFLTGTLK 130 140 150 160 170 180 LPKERLLVTV YETDDEAFNI WANEVGVPVE RIVRIGDNKG AAFASDNFWQ MSDTGPCGPC 190 200 210 220 230 240 TEIFYDHGDH IWGGPPGSPE EDGDRFIEIW NVVFMQFNRQ ADGTMEPLPR PSVDTGMGLE 250 260 270 280 290 300 RISAIMQGVH SNYEIDIFQA LIKKAAEIVG TTDLSNQSLR VIADHIRSCA FLIADGVMPS 310 320 330 340 350 360 NEGRGYVLRR IIRRAVRHGR KLGATDVFFY KLAAELAVQM KDVGAELIAQ LPLVKRVLRI 370 380 390 400 410 420 EEEQFVRTLD RGLLLLEDVL ANLGDAKVIP GEVVFKLYDT YGFPADLTAD VVREREIGID 430 440 450 460 470 480 EEGFNAEMEK QRARAKEASS FGVNYNEVLK LDFETPFTGY KQLSQKTSVV GIYKDGVEVN 490 500 510 520 530 540 GLIAGEEAVV VLAETPFYAE SGGQVGDCGV LKVDDGIFAV TDTQKAGKAI IHKGYLELGT 550 560 570 580 590 600 LEKGALVEAV VDGERRQAVA LNHSVTHLLH AALRQALGDH VTQKGSLVGA ERMRFDFSHF 610 620 630 640 650 660 EGLTMATIRR VEELVNAQIR ANHDIATQVM DLEAAKSAGA MALFGEKYED DVRVVRMGDY 670 680 690 700 710 720 STELCGGTHA KRTGDIGFFK IIAESGIAAG VRRIEAVTGK GAIDFMHQMG EQIEEAAALV 730 740 750 760 770 780 KGDQFSIADK VRQILDKSKM MERELEQLKA KLAAQAGSDL LSQVIEINGQ KVLIAALEGA 790 800 810 820 830 840 DPKSLRGMLD ELKNRMKSGV VLLATSSDDK VNLIAGVTSD LTGKVKAGEL VNLVAQQVGG 850 860 870 KGGGRPDMAQ AGGTQPEAVP AALQSVHSWL EERL

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO91771.1.
RefSeq	YP_001143519.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SS99.
Genome annotation databases
GeneID	4997680.
GenomeReviews	Gene locus ASA_3811 in contig CP000644_GR.
KEGG	asa:ASA_3811.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AERS4

Accession

Primary (citable) accession number: A4SS99

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)