Reviewed, UniProtKB/Swiss-Prot A4SS04 (KATG_AERS4)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	ASA_3715

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Catalase-peroxidase HAMAP MF_01961		PRO_0000354714
Sites
	Active site	73	1	Proton acceptor By similarity
	Metal binding	241	1	Iron (heme axial ligand) By similarity
	Site	69	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	72 ↔ 200		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-226) By similarity
	Cross-link	200 ↔ 226		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-72) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SS04-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 5B05B9F09887CBAE	FASTA	699	77,263
	10 20 30 40 50 60 MSNNSERSQG KCPVMHGGTT SGEQANMAWW PKSLNLDMAA LKQDVTALMT DSQDWWPADW 70 80 90 100 110 120 GHYGALMIRM AWHSAGTYRI ADGRGGGSTG NQRFAPLNSW PDNANLDKAR RLLWPIKKKY 130 140 150 160 170 180 GNKISWADLI ILAGTVAYES MGLKTFGFAF GREDIWHPEK DIYWGAEKEW LAPSSKPGGR 190 200 210 220 230 240 YSGERDLDNP LAAVMMGLIY VNPEGVDGNP DPLKTAKDMR VTFARMAMDD EETVALTAGG 250 260 270 280 290 300 HTVGKCHGNG DARLLGPEPE GAAVEDQGLG WLNKTQRGIG RNAVTSGIEG AWTTHPTRWD 310 320 330 340 350 360 NGYFYLLFNY EWELKKSPAG AWQWEPVNIR EEDKPVDVED PAIRHNPIMT DADMALKFDP 370 380 390 400 410 420 EYRKIAERFR ADPAAFSDAF ARAWFKLTHR DLGPKTRYVG PYVPAEDLIW QDPVPAGRTD 430 440 450 460 470 480 YDVAAVKASI AASGLSISDM VSTAWDSART FRGSDLRGGA NGARIRLAPQ NEWEGNEPAR 490 500 510 520 530 540 LARVLKVLEP IAAASQISVA DVIVLAGNLG VELAARAAGV EVTVPFSPGR GDASQARTDV 550 560 570 580 590 600 ASFDVLEPLA DGYRNWLKKD YAVTAEELML DRTQLMGLTA HEMTVLVGGM RVLGTNHGGT 610 620 630 640 650 660 RHGVFTEREG ALTNDYFVNL TDMANTWHPS GDRLYEVRDR KSGKVKWTAT RIDLVFGSNS 670 680 690 VLRAYAEVYA QDDNKEKFVC DFISAWNKVM NADRFDLMS

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO91676.1.
RefSeq	YP_001143424.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SS04.
Genome annotation databases
GeneID	4997584.
GenomeReviews	Gene locus ASA_3715 in contig CP000644_GR.
KEGG	asa:ASA_3715.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WPNALNL.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_AERS4

Accession

Primary (citable) accession number: A4SS04

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)