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Protein

Siroheme synthase 2

Gene

cysG2

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei246Proton acceptorUniRule annotation1
Active sitei268Proton donorUniRule annotation1
Binding sitei381S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei410S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 2UniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 2UniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylase 2UniRule annotation
Alternative name(s):
SUMT 2UniRule annotation
Uroporphyrinogen III methylase 2UniRule annotation
Short name:
UROM 2UniRule annotation
Precorrin-2 dehydrogenase 2UniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelatase 2UniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysG2UniRule annotation
Ordered Locus Names:ASA_3524
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003304901 – 480Siroheme synthase 2Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_3524.

Structurei

3D structure databases

ProteinModelPortaliA4SRH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST202
Regioni214 – 480Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST267
Regioni299 – 301S-adenosyl-L-methionine bindingUniRule annotation3
Regioni329 – 330S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
COG1648. LUCA.
HOGENOMiHOG000290518.
KOiK02302.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR028281. Sirohaem_synthase_central.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SRH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYLPMFARL EGRPVLLVGG GEVALRKARL LLAAGARLTL VSPEIEPEFA
60 70 80 90 100
EFAGRFTHLV ERFTPAHLAG QLLVVAATDN LEVNALVYQS ANQLGLFVNV
110 120 130 140 150
VDDPKRSSFI FPSIIDRSPL MVAVSSGGKA PVLVRLLRER LESLLPRHLG
160 170 180 190 200
GLTELSGRVR DKAKQVLSSI SDRRRFWERA FASNTLAGLI EKQDWQSAET
210 220 230 240 250
WLNDGLDKAQ SEVGEVVLVG AGPGDPGLLT LKALQQIQQA EVVLYDQLVS
260 270 280 290 300
PEILDLVRRD ATLVSVGKKA GAHSVPQEET NRLLVEYARA GNRVVRLKGG
310 320 330 340 350
DPFMFGCGGE ELEVLAAEGI PFSVVPGITA AAGATAYAGI PLTHRDHAQS
360 370 380 390 400
AVFITGHCQK EGKEPDWQQL AVTQQTLVIY MGLMRSEHIQ QQLVSHGRSC
410 420 430 440 450
TTPIAIIEWG TTARQRVLTG TLAELAVLAQ QAVSPSLIVI GEVVSLRKQL
460 470 480
AWFGEKSGEK RDVQAGTDMN GCDLKLVNLA
Length:480
Mass (Da):51,976
Last modified:April 29, 2008 - v2
Checksum:i73EA4371971ABAA8
GO

Sequence cautioni

The sequence ABO91492 differs from that shown. Reason: Frameshift at position 22.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO91492.1. Frameshift.

Genome annotation databases

EnsemblBacteriaiABO91492; ABO91492; ASA_3524.
KEGGiasa:ASA_3524.
PATRICi20793502. VBIAerSal2987_3511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO91492.1. Frameshift.

3D structure databases

ProteinModelPortaliA4SRH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_3524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO91492; ABO91492; ASA_3524.
KEGGiasa:ASA_3524.
PATRICi20793502. VBIAerSal2987_3511.

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
COG1648. LUCA.
HOGENOMiHOG000290518.
KOiK02302.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR028281. Sirohaem_synthase_central.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSG2_AERS4
AccessioniPrimary (citable) accession number: A4SRH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.