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A4SRH0

- CYSG2_AERS4

UniProt

A4SRH0 - CYSG2_AERS4

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Protein
Siroheme synthase 2
Gene
cysG2, ASA_3524
Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei246 – 2461Proton acceptor By similarity
Active sitei268 – 2681Proton donor By similarity
Binding sitei381 – 3811S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei410 – 4101S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASAL382245:GJJN-3511-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 2
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 2 (EC:2.1.1.107)
Short name:
Urogen III methylase 2
Alternative name(s):
SUMT 2
Uroporphyrinogen III methylase 2
Short name:
UROM 2
Precorrin-2 dehydrogenase 2 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 2 (EC:4.99.1.4)
Gene namesi
Name:cysG2
Ordered Locus Names:ASA_3524
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000225: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Siroheme synthase 2UniRule annotation
PRO_0000330490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei126 – 1261Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_3524.

Structurei

3D structure databases

ProteinModelPortaliA4SRH0.
SMRiA4SRH0. Positions 1-455.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 202202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Add
BLAST
Regioni214 – 480267Uroporphyrinogen-III C-methyltransferase By similarity
Add
BLAST
Regioni299 – 3013S-adenosyl-L-methionine binding By similarity
Regioni329 – 3302S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OrthoDBiEOG6BGNXZ.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SRH0-1 [UniParc]FASTAAdd to Basket

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MDYLPMFARL EGRPVLLVGG GEVALRKARL LLAAGARLTL VSPEIEPEFA    50
EFAGRFTHLV ERFTPAHLAG QLLVVAATDN LEVNALVYQS ANQLGLFVNV 100
VDDPKRSSFI FPSIIDRSPL MVAVSSGGKA PVLVRLLRER LESLLPRHLG 150
GLTELSGRVR DKAKQVLSSI SDRRRFWERA FASNTLAGLI EKQDWQSAET 200
WLNDGLDKAQ SEVGEVVLVG AGPGDPGLLT LKALQQIQQA EVVLYDQLVS 250
PEILDLVRRD ATLVSVGKKA GAHSVPQEET NRLLVEYARA GNRVVRLKGG 300
DPFMFGCGGE ELEVLAAEGI PFSVVPGITA AAGATAYAGI PLTHRDHAQS 350
AVFITGHCQK EGKEPDWQQL AVTQQTLVIY MGLMRSEHIQ QQLVSHGRSC 400
TTPIAIIEWG TTARQRVLTG TLAELAVLAQ QAVSPSLIVI GEVVSLRKQL 450
AWFGEKSGEK RDVQAGTDMN GCDLKLVNLA 480
Length:480
Mass (Da):51,976
Last modified:April 29, 2008 - v2
Checksum:i73EA4371971ABAA8
GO

Sequence cautioni

The sequence ABO91492.1 differs from that shown. Reason: Frameshift at position 22.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO91492.1. Frameshift.
RefSeqiYP_001143240.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO91492; ABO91492; ASA_3524.
GeneIDi4995755.
KEGGiasa:ASA_3524.
PATRICi20793502. VBIAerSal2987_3511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO91492.1 . Frameshift.
RefSeqi YP_001143240.1. NC_009348.1.

3D structure databases

ProteinModelPortali A4SRH0.
SMRi A4SRH0. Positions 1-455.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 382245.ASA_3524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO91492 ; ABO91492 ; ASA_3524 .
GeneIDi 4995755.
KEGGi asa:ASA_3524.
PATRICi 20793502. VBIAerSal2987_3511.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OrthoDBi EOG6BGNXZ.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci ASAL382245:GJJN-3511-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449.

Entry informationi

Entry nameiCYSG2_AERS4
AccessioniPrimary (citable) accession number: A4SRH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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