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Protein

Siroheme synthase 2

Gene

cysG2

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase 2 (cysG2), Siroheme synthase 1 (cysG1)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei246Proton acceptorUniRule annotation1
Active sitei268Proton donorUniRule annotation1
Binding sitei381S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei410S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Methyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandNAD, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211
UPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 2UniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 2UniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylase 2UniRule annotation
Alternative name(s):
SUMT 2UniRule annotation
Uroporphyrinogen III methylase 2UniRule annotation
Short name:
UROM 2UniRule annotation
Precorrin-2 dehydrogenase 2UniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelatase 2UniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysG2UniRule annotation
Ordered Locus Names:ASA_3524
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003304901 – 480Siroheme synthase 2Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_3524

Structurei

3D structure databases

ProteinModelPortaliA4SRH0
SMRiA4SRH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST202
Regioni214 – 480Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST267
Regioni299 – 301S-adenosyl-L-methionine bindingUniRule annotation3
Regioni329 – 330S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
COG1648 LUCA
HOGENOMiHOG000290518
KOiK02302

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR028281 Sirohaem_synthase_central
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF14824 Sirohm_synth_M, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

A4SRH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYLPMFARL EGRPVLLVGG GEVALRKARL LLAAGARLTL VSPEIEPEFA
60 70 80 90 100
EFAGRFTHLV ERFTPAHLAG QLLVVAATDN LEVNALVYQS ANQLGLFVNV
110 120 130 140 150
VDDPKRSSFI FPSIIDRSPL MVAVSSGGKA PVLVRLLRER LESLLPRHLG
160 170 180 190 200
GLTELSGRVR DKAKQVLSSI SDRRRFWERA FASNTLAGLI EKQDWQSAET
210 220 230 240 250
WLNDGLDKAQ SEVGEVVLVG AGPGDPGLLT LKALQQIQQA EVVLYDQLVS
260 270 280 290 300
PEILDLVRRD ATLVSVGKKA GAHSVPQEET NRLLVEYARA GNRVVRLKGG
310 320 330 340 350
DPFMFGCGGE ELEVLAAEGI PFSVVPGITA AAGATAYAGI PLTHRDHAQS
360 370 380 390 400
AVFITGHCQK EGKEPDWQQL AVTQQTLVIY MGLMRSEHIQ QQLVSHGRSC
410 420 430 440 450
TTPIAIIEWG TTARQRVLTG TLAELAVLAQ QAVSPSLIVI GEVVSLRKQL
460 470 480
AWFGEKSGEK RDVQAGTDMN GCDLKLVNLA
Length:480
Mass (Da):51,976
Last modified:April 29, 2008 - v2
Checksum:i73EA4371971ABAA8
GO

Sequence cautioni

The sequence ABO91492 differs from that shown. Reason: Frameshift at position 22.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA Translation: ABO91492.1 Frameshift.

Genome annotation databases

EnsemblBacteriaiABO91492; ABO91492; ASA_3524
KEGGiasa:ASA_3524

Similar proteinsi

Entry informationi

Entry nameiCYSG2_AERS4
AccessioniPrimary (citable) accession number: A4SRH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: March 28, 2018
This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health