A4SRF2 (E4PD_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-erythrose-4-phosphate dehydrogenase
Short name=E4PDH
EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	ASA_3506

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity. HAMAP MF_01640
Catalytic activity	D-erythrose 4-phosphate + NAD+ + H2O = 4-phosphoerythronate + NADH. HAMAP MF_01640
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5. HAMAP MF_01640
Subunit structure	Homotetramer By similarity. HAMAP MF_01640
Subcellular location	Cytoplasm By similarity HAMAP MF_01640.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: InterPro pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	D-erythrose-4-phosphate dehydrogenase HAMAP MF_01640		PRO_0000293144
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	153 – 155	3	Substrate binding Potential
	Region	212 – 213	2	Substrate binding Potential
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	199	1	Substrate Potential
	Binding site	235	1	Substrate Potential
	Binding site	317	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SRF2 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E8921487B5FB191C	FASTA	336	36,786
	10 20 30 40 50 60 MIKIAINGYG RIGRNVLRAL YESGRDKNIK IVAINELAAP EAMVHLTRFD TSHGRFRYPV 70 80 90 100 110 120 QLAGHSMLVG EDLISLFAEQ DPANLPWRAL GVDVVLDCTG VFGSRADAEL HLGAGAGKVL 130 140 150 160 170 180 FSHPADADVD ATIVYGVNHQ VLTGRERIVS NASCTTNCVV PVIETLHREF EINCGTITTI 190 200 210 220 230 240 HSAMHDQQVI DAYHSDLRRT RAASQSIIPV DTKLAKGLER ILPHFAGKFE AIAVRVPTIN 250 260 270 280 290 300 VTAMDLSITV RKKVTVTDVN QALQRASRGT LSGILDYTEE PLVSVDFNHD AHSCIIDGTQ 310 320 330 TRVSDANLVK MLMWCDNEWG FANRMLDTTR AMMAAG

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91474.1.
RefSeq	YP_001143222.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SRF2.
SMR	A4SRF2. Positions 2-335.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SRF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4997757.
GenomeReviews	Gene locus ASA_3506 in contig CP000644_GR.
KEGG	asa:ASA_3506.
PATRIC	20793468. VBIAerSal2987_3494.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0057.
HOGENOM	HBG571736.
OMA	TTHGRFQ.
PhylomeDB	A4SRF2.
ProtClustDB	PRK13535.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_3506-MONOMER.
Family and domain databases
HAMAP	MF_01640. E4P_dehydrog. [Tree]
InterPro	IPR006422. E4P_DH_bac. IPR020831. GlycerAld/Erythrose_P_DH. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K03472.
PANTHER	PTHR10836. GAP_DH. 1 hit. PTHR10836:SF24. PTHR10836:SF24. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_AERS4

Accession

Primary (citable) accession number: A4SRF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program