A4SRB9 (A4SRB9_AERS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108 EMBL ABO91441.1
Ordered Locus Names:	ASA_3473

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ABO91441.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	21	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	28	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	155	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	211	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	A4SRB9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E10232070557B9FB	FASTA	229	24,608
	10 20 30 40 50 60 MTDVKITGVI AIVPAAGIGS RMRADCPKQY LHLAGKTILE HTLERLLSHP AIAQVIVALA 70 80 90 100 110 120 PHDRWFETLP VAADSRILRV EGGAERAFSV LNALHAADSD WVLVHDAARP CLTHGDLDAL 130 140 150 160 170 180 IAAAIQCGGA ILGSPVRDTM KRSDAAGNII GTVDRELLWH ALTPQMFPAR TLKRALEEEL 190 200 210 220 ALGATITDEA SAMERAGFTV RMVEGRADNI KVTRPEDLSL AGLFLSQQT

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91441.1.
RefSeq	YP_001143189.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SRB9.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SRB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4995580.
GenomeReviews	Gene locus ASA_3473 in contig CP000644_GR.
KEGG	asa:ASA_3473.
PATRIC	20793402. VBIAerSal2987_3461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	FPQGAIL.
PhylomeDB	A4SRB9.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4SRB9_AERS4

Accession

Primary (citable) accession number: A4SRB9

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)