Reviewed, UniProtKB/Swiss-Prot A4SQM2 (RLMG_AERS4)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	ASA_3201

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366445

Sequences

	Sequence		Length	Mass (Da)
	A4SQM2-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 50F023C7CEFB6565	FASTA	377	41,548
	10 20 30 40 50 60 MQTLLDTAHC RLTLYRYPRQ SQDPLQAWDA ADEYLINTLA ETPLEQAGPV IIMNDGFGAL 70 80 90 100 110 120 AAFLHPHAPV CVSDSYISER ATLANLAENE LDPHAIRLQD ALAPLPLAPA LVVIKVSKYQ 130 140 150 160 170 180 ALLEQQLLAL RAVVTPATRV IAAGKAKDIH SSTLALFEKY LGPTRTSLAW KKARLIHCEP 190 200 210 220 230 240 QAMQPELANP YPTVWPLEGT GMLIHNHANV FSRTSLDIGA RFMLDNLPVH SARKVIDLGC 250 260 270 280 290 300 GNGVLGLALL AKDPEVEVTF IDESHMAVAS ARLNVEHNLP DALPRARFMV NNCLDDVAVG 310 320 330 340 350 360 AADRILCNPP FHQLQAITDH IAWQMFSDAH RVLPQGGELW IVGNRHLDYH NKLKRLFANA 370 QVVASNSKFV ILKAIKR

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91194.1. Different initiation.
RefSeq	YP_001142942.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SQM2.
Genome annotation databases
GeneID	4998207.
GenomeReviews	Gene locus ASA_3201 in contig CP000644_GR.
KEGG	asa:ASA_3201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
PhylomeDB	A4SQM2.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_AERS4

Accession

Primary (citable) accession number: A4SQM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 19, 2010
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)