A4SQM2 (RLMG_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	ASA_3201

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABO91194.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366445

Sequences

	Sequence		Length	Mass (Da)
	A4SQM2 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 50F023C7CEFB6565	FASTA	377	41,548
	10 20 30 40 50 60 MQTLLDTAHC RLTLYRYPRQ SQDPLQAWDA ADEYLINTLA ETPLEQAGPV IIMNDGFGAL 70 80 90 100 110 120 AAFLHPHAPV CVSDSYISER ATLANLAENE LDPHAIRLQD ALAPLPLAPA LVVIKVSKYQ 130 140 150 160 170 180 ALLEQQLLAL RAVVTPATRV IAAGKAKDIH SSTLALFEKY LGPTRTSLAW KKARLIHCEP 190 200 210 220 230 240 QAMQPELANP YPTVWPLEGT GMLIHNHANV FSRTSLDIGA RFMLDNLPVH SARKVIDLGC 250 260 270 280 290 300 GNGVLGLALL AKDPEVEVTF IDESHMAVAS ARLNVEHNLP DALPRARFMV NNCLDDVAVG 310 320 330 340 350 360 AADRILCNPP FHQLQAITDH IAWQMFSDAH RVLPQGGELW IVGNRHLDYH NKLKRLFANA 370 QVVASNSKFV ILKAIKR

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91194.1. Different initiation.
RefSeq	YP_001142942.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SQM2.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SQM2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4998207.
GenomeReviews	Gene locus ASA_3201 in contig CP000644_GR.
KEGG	asa:ASA_3201.
PATRIC	20792817. VBIAerSal2987_3179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
ProtClustDB	CLSK2504743.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_3201-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_AERS4

Accession

Primary (citable) accession number: A4SQM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program