A4SQJ9 (QUEF_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	ASA_3178

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000193211

Sequences

	Sequence		Length	Mass (Da)
	A4SQJ9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A7C8A1ABD1D3F8C6	FASTA	282	31,607
	10 20 30 40 50 60 MSKQEKYAGA SALQGLSLGQ QSAYISQYSP ALLQPVPRSL NRDDLGLTGE LPFQGCDVWT 70 80 90 100 110 120 LYELSWLNAR GKPMVAVGEV SVPAVSANLI ESKSFKLYLN SFNQTRCDSL EAVQAMLVKD 130 140 150 160 170 180 LSACAGSEVS VTLFPLAQAP HHIAALPGEC IDEQDIEVDC YEFDANLLQG AAGNDEVEET 190 200 210 220 230 240 LHSHLLKSNC LVTSQPDWGS VVIHYRGPRL DREKLLRYLI SFRQHNEFHE QCIERIFTDL 250 260 270 280 KLLCHPSQLT VYARYTRRGG LDINPFRSDW ELPPTNLRLI RQ

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO91171.1.
RefSeq	YP_001142919.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SQJ9.
SMR	A4SQJ9. Positions 24-282.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SQJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4995889.
GenomeReviews	Gene locus ASA_3178 in contig CP000644_GR.
KEGG	asa:ASA_3178.
PATRIC	20792769. VBIAerSal2987_3155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
PhylomeDB	A4SQJ9.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_3178-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_AERS4

Accession

Primary (citable) accession number: A4SQJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program