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A4SQD3 (A4SQD3_AERS4) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1 HAMAP-Rule MF_00952

EC=5.99.1.2 HAMAP-Rule MF_00952
Alternative name(s):
DNA topoisomerase I HAMAP-Rule MF_00952
Gene names
Name:topA HAMAP-Rule MF_00952 EMBL ABO91105.1
Ordered Locus Names:ASA_3111 EMBL ABO91105.1
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP] EMBL ABO91105.1
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952 SAAS SAAS005733

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952 SAAS SAAS005733

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family. RuleBase RU004206 HAMAP-Rule MF_00952

Contains 1 Toprim domain. HAMAP-Rule MF_00952

Contains Toprim domain. SAAS SAAS005733

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain3 – 145143Toprim By similarity HAMAP-Rule MF_00952
Region195 – 2006Interaction with DNA By similarity HAMAP-Rule MF_00952

Sites

Active site3221O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_00952
Metal binding91Magnesium 1; catalytic By similarity HAMAP-Rule MF_00952
Metal binding1141Magnesium 1; catalytic By similarity HAMAP-Rule MF_00952
Metal binding1141Magnesium 2 By similarity HAMAP-Rule MF_00952
Metal binding1161Magnesium 2 By similarity HAMAP-Rule MF_00952
Site331Interaction with DNA By similarity HAMAP-Rule MF_00952
Site1711Interaction with DNA By similarity HAMAP-Rule MF_00952
Site1721Interaction with DNA By similarity HAMAP-Rule MF_00952
Site1751Interaction with DNA By similarity HAMAP-Rule MF_00952
Site1801Interaction with DNA By similarity HAMAP-Rule MF_00952
Site1871Interaction with DNA By similarity HAMAP-Rule MF_00952
Site3241Interaction with DNA By similarity HAMAP-Rule MF_00952
Site5101Interaction with DNA By similarity HAMAP-Rule MF_00952

Sequences

Sequence LengthMass (Da)Tools
A4SQD3 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 7D58BDFCD322E355

FASTA87396,991
        10         20         30         40         50         60 
MGKSLVIVES PAKAKTINKY LGKDYVVKSS VGHVRDLPTS GSGSNEPKKP VIRGIKLSES 

        70         80         90        100        110        120 
EKAAKDRNAL FSRMGINPTA GWQANYQILP GKEKVVSELQ SLAAKADTIY LATDLDREGE 

       130        140        150        160        170        180 
AIAWHLREII GGDDSRYKRV VFNEITKTAI QEAFAQPSEL NIHRVNAQQA RRFLDRVVGY 

       190        200        210        220        230        240 
MVSPLLWKKL ARGLSAGRVQ SVAVRLIVDK EREIKAFVPV EFWDLNADLL TGDKSDLRME 

       250        260        270        280        290        300 
VVSRNGADFK PVTQAETFAA VAALEGVDYK VVNREDKPTG SKPSAPFITS TLQQAASTRL 

       310        320        330        340        350        360 
SFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SKEAVEALRE YIGEQYGAAY LPAEPNLYGS 

       370        380        390        400        410        420 
KAGAQEAHEA IRPSSVLVTA EMLEGMEPDA MRLYDLIWRQ FVACQMTPAQ YDSSTLTVKA 

       430        440        450        460        470        480 
GEFELKARGR TLRFAGWTKA LPPMGRKGED SQLPAVTVGE VLKLIKLDPR QHFTKPPARF 

       490        500        510        520        530        540 
TEAALVRELE KRGIGRPSTY ASIISTIVDR GYVRVESRRF FAEKMGEIVT DRLVENFVEL 

       550        560        570        580        590        600 
MNYEFTAKME DKLDGIAEGS LEWKKVLDAF YEEFTQELAK ADRDAEEGGM RANQMVLTDI 

       610        620        630        640        650        660 
DCPDCGRKMG IRTATTGVFL GCSGYALPPK ERCKKTINLV SADEFVSATD GDEAETEALR 

       670        680        690        700        710        720 
AKHRCGVCGT AMDAYIIDDS RKLHVCGRNP DCDGYEIEKG QFKLKGYEGP SIECDRCGSE 

       730        740        750        760        770        780 
MQLKNGRFGK YMGCTNETCK NTRKILKNGD IAPPKEDPVP LPELKCTQSD AYFVLRDGAA 

       790        800        810        820        830        840 
GLFLAASNFP KSRETRAPLV EELKRFKERL SPKHQYLADA PASDPDGNAS IVRFSRKTKE 

       850        860        870 
QYVMTEVNGK ATGWTAHYDN GKWQESLAKS KKA 

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References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000644 Genomic DNA. Translation: ABO91105.1.
RefSeqYP_001142853.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4SQD3.
SMRA4SQD3. Positions 2-595, 748-865.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382245.ASA_3111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO91105; ABO91105; ASA_3111.
GeneID4995997.
KEGGasa:ASA_3111.
PATRIC20792637. VBIAerSal2987_3089.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK07561.

Enzyme and pathway databases

BioCycASAL382245:GJJN-3098-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA4SQD3_AERS4
AccessionPrimary (citable) accession number: A4SQD3
Entry history
Integrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)