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A4SQD3

- A4SQD3_AERS4

UniProt

A4SQD3 - A4SQD3_AERS4

Protein

DNA topoisomerase 1

Gene

topA

Organism
Aeromonas salmonicida (strain A449)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi114 – 1141Magnesium 1; catalyticUniRule annotation
    Metal bindingi114 – 1141Magnesium 2UniRule annotation
    Metal bindingi116 – 1161Magnesium 2UniRule annotation
    Sitei171 – 1711Interaction with DNAUniRule annotation
    Sitei172 – 1721Interaction with DNAUniRule annotation
    Sitei175 – 1751Interaction with DNAUniRule annotation
    Sitei180 – 1801Interaction with DNAUniRule annotation
    Sitei187 – 1871Interaction with DNAUniRule annotation
    Active sitei322 – 3221O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei324 – 3241Interaction with DNAUniRule annotation
    Sitei510 – 5101Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
    5. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, TopoisomeraseUniRule annotationSAAS annotation

    Keywords - Ligandi

    DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciASAL382245:GJJN-3098-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Gene namesi
    Name:topAUniRule annotationImported
    Ordered Locus Names:ASA_3111Imported
    OrganismiAeromonas salmonicida (strain A449)Imported
    Taxonomic identifieri382245 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
    ProteomesiUP000000225: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi382245.ASA_3111.

    Structurei

    3D structure databases

    ProteinModelPortaliA4SQD3.
    SMRiA4SQD3. Positions 2-595, 748-865.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 145143ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni195 – 2006Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation
    Contains Toprim domain.SAAS annotation

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4SQD3-1 [UniParc]FASTAAdd to Basket

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    MGKSLVIVES PAKAKTINKY LGKDYVVKSS VGHVRDLPTS GSGSNEPKKP    50
    VIRGIKLSES EKAAKDRNAL FSRMGINPTA GWQANYQILP GKEKVVSELQ 100
    SLAAKADTIY LATDLDREGE AIAWHLREII GGDDSRYKRV VFNEITKTAI 150
    QEAFAQPSEL NIHRVNAQQA RRFLDRVVGY MVSPLLWKKL ARGLSAGRVQ 200
    SVAVRLIVDK EREIKAFVPV EFWDLNADLL TGDKSDLRME VVSRNGADFK 250
    PVTQAETFAA VAALEGVDYK VVNREDKPTG SKPSAPFITS TLQQAASTRL 300
    SFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SKEAVEALRE YIGEQYGAAY 350
    LPAEPNLYGS KAGAQEAHEA IRPSSVLVTA EMLEGMEPDA MRLYDLIWRQ 400
    FVACQMTPAQ YDSSTLTVKA GEFELKARGR TLRFAGWTKA LPPMGRKGED 450
    SQLPAVTVGE VLKLIKLDPR QHFTKPPARF TEAALVRELE KRGIGRPSTY 500
    ASIISTIVDR GYVRVESRRF FAEKMGEIVT DRLVENFVEL MNYEFTAKME 550
    DKLDGIAEGS LEWKKVLDAF YEEFTQELAK ADRDAEEGGM RANQMVLTDI 600
    DCPDCGRKMG IRTATTGVFL GCSGYALPPK ERCKKTINLV SADEFVSATD 650
    GDEAETEALR AKHRCGVCGT AMDAYIIDDS RKLHVCGRNP DCDGYEIEKG 700
    QFKLKGYEGP SIECDRCGSE MQLKNGRFGK YMGCTNETCK NTRKILKNGD 750
    IAPPKEDPVP LPELKCTQSD AYFVLRDGAA GLFLAASNFP KSRETRAPLV 800
    EELKRFKERL SPKHQYLADA PASDPDGNAS IVRFSRKTKE QYVMTEVNGK 850
    ATGWTAHYDN GKWQESLAKS KKA 873
    Length:873
    Mass (Da):96,991
    Last modified:May 15, 2007 - v1
    Checksum:i7D58BDFCD322E355
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000644 Genomic DNA. Translation: ABO91105.1.
    RefSeqiYP_001142853.1. NC_009348.1.

    Genome annotation databases

    EnsemblBacteriaiABO91105; ABO91105; ASA_3111.
    GeneIDi4995997.
    KEGGiasa:ASA_3111.
    PATRICi20792637. VBIAerSal2987_3089.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000644 Genomic DNA. Translation: ABO91105.1 .
    RefSeqi YP_001142853.1. NC_009348.1.

    3D structure databases

    ProteinModelPortali A4SQD3.
    SMRi A4SQD3. Positions 2-595, 748-865.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 382245.ASA_3111.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO91105 ; ABO91105 ; ASA_3111 .
    GeneIDi 4995997.
    KEGGi asa:ASA_3111.
    PATRICi 20792637. VBIAerSal2987_3089.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci ASAL382245:GJJN-3098-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
      Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
      BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: A449Imported.

    Entry informationi

    Entry nameiA4SQD3_AERS4
    AccessioniPrimary (citable) accession number: A4SQD3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 15, 2007
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3