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Protein

DNA topoisomerase 1

Gene

topA

Organism
Aeromonas salmonicida (strain A449)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei322O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Isomerase, TopoisomeraseUniRule annotationSAAS annotation
LigandMagnesiumSAAS annotation, Metal-bindingSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Gene namesi
Name:topAUniRule annotationImported
Ordered Locus Names:ASA_3111Imported
OrganismiAeromonas salmonicida (strain A449)Imported
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with DNAUniRule annotation1
Sitei171Interaction with DNAUniRule annotation1
Sitei172Interaction with DNAUniRule annotation1
Sitei175Interaction with DNAUniRule annotation1
Sitei180Interaction with DNAUniRule annotation1
Sitei187Interaction with DNAUniRule annotation1
Sitei324Interaction with DNAUniRule annotation1
Sitei510Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi382245.ASA_3111

Structurei

3D structure databases

ProteinModelPortaliA4SQD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 145ToprimInterPro annotationAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 200Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG0551 LUCA
HOGENOMiHOG000004018
KOiK03168
OMAiPECKYTR

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR013263 TopoI_Znr_bac
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF08272 Topo_Zn_Ribbon, 2 hits
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

A4SQD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSLVIVES PAKAKTINKY LGKDYVVKSS VGHVRDLPTS GSGSNEPKKP
60 70 80 90 100
VIRGIKLSES EKAAKDRNAL FSRMGINPTA GWQANYQILP GKEKVVSELQ
110 120 130 140 150
SLAAKADTIY LATDLDREGE AIAWHLREII GGDDSRYKRV VFNEITKTAI
160 170 180 190 200
QEAFAQPSEL NIHRVNAQQA RRFLDRVVGY MVSPLLWKKL ARGLSAGRVQ
210 220 230 240 250
SVAVRLIVDK EREIKAFVPV EFWDLNADLL TGDKSDLRME VVSRNGADFK
260 270 280 290 300
PVTQAETFAA VAALEGVDYK VVNREDKPTG SKPSAPFITS TLQQAASTRL
310 320 330 340 350
SFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SKEAVEALRE YIGEQYGAAY
360 370 380 390 400
LPAEPNLYGS KAGAQEAHEA IRPSSVLVTA EMLEGMEPDA MRLYDLIWRQ
410 420 430 440 450
FVACQMTPAQ YDSSTLTVKA GEFELKARGR TLRFAGWTKA LPPMGRKGED
460 470 480 490 500
SQLPAVTVGE VLKLIKLDPR QHFTKPPARF TEAALVRELE KRGIGRPSTY
510 520 530 540 550
ASIISTIVDR GYVRVESRRF FAEKMGEIVT DRLVENFVEL MNYEFTAKME
560 570 580 590 600
DKLDGIAEGS LEWKKVLDAF YEEFTQELAK ADRDAEEGGM RANQMVLTDI
610 620 630 640 650
DCPDCGRKMG IRTATTGVFL GCSGYALPPK ERCKKTINLV SADEFVSATD
660 670 680 690 700
GDEAETEALR AKHRCGVCGT AMDAYIIDDS RKLHVCGRNP DCDGYEIEKG
710 720 730 740 750
QFKLKGYEGP SIECDRCGSE MQLKNGRFGK YMGCTNETCK NTRKILKNGD
760 770 780 790 800
IAPPKEDPVP LPELKCTQSD AYFVLRDGAA GLFLAASNFP KSRETRAPLV
810 820 830 840 850
EELKRFKERL SPKHQYLADA PASDPDGNAS IVRFSRKTKE QYVMTEVNGK
860 870
ATGWTAHYDN GKWQESLAKS KKA
Length:873
Mass (Da):96,991
Last modified:May 15, 2007 - v1
Checksum:i7D58BDFCD322E355
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA Translation: ABO91105.1
RefSeqiWP_005312182.1, NC_009348.1

Genome annotation databases

EnsemblBacteriaiABO91105; ABO91105; ASA_3111
GeneIDi4995997
KEGGiasa:ASA_3111
PATRICifig|382245.13.peg.3089

Similar proteinsi

Entry informationi

Entry nameiA4SQD3_AERS4
AccessioniPrimary (citable) accession number: A4SQD3
Entry historyiIntegrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: June 20, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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