Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A4SQD3

- A4SQD3_AERS4

UniProt

A4SQD3 - A4SQD3_AERS4

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA topoisomerase 1

Gene

topA

Organism
Aeromonas salmonicida (strain A449)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotationSAAS annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi114 – 1141Magnesium 1; catalyticUniRule annotation
Metal bindingi114 – 1141Magnesium 2UniRule annotation
Metal bindingi116 – 1161Magnesium 2UniRule annotation
Sitei171 – 1711Interaction with DNAUniRule annotation
Sitei172 – 1721Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Sitei180 – 1801Interaction with DNAUniRule annotation
Sitei187 – 1871Interaction with DNAUniRule annotation
Active sitei322 – 3221O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei324 – 3241Interaction with DNAUniRule annotation
Sitei510 – 5101Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotation

Keywords - Ligandi

DNA-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciASAL382245:GJJN-3098-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Gene namesi
Name:topAUniRule annotationImported
Ordered Locus Names:ASA_3111Imported
OrganismiAeromonas salmonicida (strain A449)Imported
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000225: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi382245.ASA_3111.

Structurei

3D structure databases

ProteinModelPortaliA4SQD3.
SMRiA4SQD3. Positions 2-595, 748-865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 145143ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni195 – 2006Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotation

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SQD3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKSLVIVES PAKAKTINKY LGKDYVVKSS VGHVRDLPTS GSGSNEPKKP
60 70 80 90 100
VIRGIKLSES EKAAKDRNAL FSRMGINPTA GWQANYQILP GKEKVVSELQ
110 120 130 140 150
SLAAKADTIY LATDLDREGE AIAWHLREII GGDDSRYKRV VFNEITKTAI
160 170 180 190 200
QEAFAQPSEL NIHRVNAQQA RRFLDRVVGY MVSPLLWKKL ARGLSAGRVQ
210 220 230 240 250
SVAVRLIVDK EREIKAFVPV EFWDLNADLL TGDKSDLRME VVSRNGADFK
260 270 280 290 300
PVTQAETFAA VAALEGVDYK VVNREDKPTG SKPSAPFITS TLQQAASTRL
310 320 330 340 350
SFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SKEAVEALRE YIGEQYGAAY
360 370 380 390 400
LPAEPNLYGS KAGAQEAHEA IRPSSVLVTA EMLEGMEPDA MRLYDLIWRQ
410 420 430 440 450
FVACQMTPAQ YDSSTLTVKA GEFELKARGR TLRFAGWTKA LPPMGRKGED
460 470 480 490 500
SQLPAVTVGE VLKLIKLDPR QHFTKPPARF TEAALVRELE KRGIGRPSTY
510 520 530 540 550
ASIISTIVDR GYVRVESRRF FAEKMGEIVT DRLVENFVEL MNYEFTAKME
560 570 580 590 600
DKLDGIAEGS LEWKKVLDAF YEEFTQELAK ADRDAEEGGM RANQMVLTDI
610 620 630 640 650
DCPDCGRKMG IRTATTGVFL GCSGYALPPK ERCKKTINLV SADEFVSATD
660 670 680 690 700
GDEAETEALR AKHRCGVCGT AMDAYIIDDS RKLHVCGRNP DCDGYEIEKG
710 720 730 740 750
QFKLKGYEGP SIECDRCGSE MQLKNGRFGK YMGCTNETCK NTRKILKNGD
760 770 780 790 800
IAPPKEDPVP LPELKCTQSD AYFVLRDGAA GLFLAASNFP KSRETRAPLV
810 820 830 840 850
EELKRFKERL SPKHQYLADA PASDPDGNAS IVRFSRKTKE QYVMTEVNGK
860 870
ATGWTAHYDN GKWQESLAKS KKA
Length:873
Mass (Da):96,991
Last modified:May 15, 2007 - v1
Checksum:i7D58BDFCD322E355
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO91105.1.
RefSeqiYP_001142853.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO91105; ABO91105; ASA_3111.
GeneIDi4995997.
KEGGiasa:ASA_3111.
PATRICi20792637. VBIAerSal2987_3089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO91105.1 .
RefSeqi YP_001142853.1. NC_009348.1.

3D structure databases

ProteinModelPortali A4SQD3.
SMRi A4SQD3. Positions 2-595, 748-865.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 382245.ASA_3111.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO91105 ; ABO91105 ; ASA_3111 .
GeneIDi 4995997.
KEGGi asa:ASA_3111.
PATRICi 20792637. VBIAerSal2987_3089.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci ASAL382245:GJJN-3098-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449Imported.

Entry informationi

Entry nameiA4SQD3_AERS4
AccessioniPrimary (citable) accession number: A4SQD3
Entry historyi
Integrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3