Reviewed, UniProtKB/Swiss-Prot A4SQ71 (ACEK_AERS4)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	ASA_3042

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000046547
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SQ71-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E689E71ADD3453C4	FASTA	575	67,385
	10 20 30 40 50 60 MQQPLEQAVA ETILQRFESF YSRFLEITQG SKSRFENSDW LGVQLAGRER IRLYDHHVGA 70 80 90 100 110 120 TTALIKQMMG EHHATQELLK RVKGAFSDLL PRCDNFEVAE SFFNSVYRRI FRHRNIRDEN 130 140 150 160 170 180 LYIHPFRSRG EHPDLSALLR VYRTDLTHLP QTLSQMLGDY SFTLPYEDKQ RDIVDIHRHL 190 200 210 220 230 240 AENGPQILHE EPFAIELLKE VFYRNKGAYL VGLIRVKGQV FPFILPLLST GQSIYVDTVI 250 260 270 280 290 300 FEPELASIVV GFARAYFMVY APVPALFVVF LRQIMPHKPD YEIYNAIGCQ KHGKTELYRH 310 320 330 340 350 360 YQQHLAQSRE QFVIAPGIKG MVMSVFTLPS YDVVFKVIKD EFTPPKDVSH EQVRAKYRLV 370 380 390 400 410 420 KQHDRVGRMA DTQEFTNFEF PLDRISPELL AELKTVAPSA LTITSDKLVI KHLYTERKMI 430 440 450 460 470 480 PLNLYLDKAD EQQTRLALEE YGNAIKQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTECNFRQI PPPRYPEDEW SAEPWYSVAP NDIFPEEFAT FLLQKPQVRE IMMQLHKEMF 550 560 570 DANYWKMLQS NIKVGVFEDV YPYRRKKRFK FQRGG

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO91043.1.
RefSeq	YP_001142791.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SQ71.
Genome annotation databases
GeneID	4995719.
GenomeReviews	Gene locus ASA_3042 in contig CP000644_GR.
KEGG	asa:ASA_3042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_AERS4

Accession

Primary (citable) accession number: A4SQ71

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)