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Protein

NAD kinase

Gene

nadK

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Proton acceptorUniRule annotation
Binding sitei158 – 1581NADUniRule annotation
Binding sitei175 – 1751NADUniRule annotation
Binding sitei177 – 1771NADUniRule annotation
Binding sitei249 – 2491NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742NADUniRule annotation
Nucleotide bindingi147 – 1482NADUniRule annotation
Nucleotide bindingi188 – 1936NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciASAL382245:GJJN-2985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:ASA_2998
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000225: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294NAD kinasePRO_1000079478Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_2998.

Structurei

3D structure databases

ProteinModelPortaliA4SQ27.
SMRiA4SQ27. Positions 4-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A4SQ27-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSPFKTIAL IGKPHHEGAN QTLTGLHQYL TTRGFKVLVE SRVAHTLGIM
60 70 80 90 100
DENVMDLVQL GEQADLAIVV GGDGNMLGAA RVLSRFDVAV IGVNRGNLGF
110 120 130 140 150
LTDLSPQDYL LPLEQVLSGH YKSEHRFLLE AAVYRHGERK SSNLAVNEAV
160 170 180 190 200
LHPGKIAHMI EFEVYIDGSF MYSQRSDGII VATPTGSTAY SLSAGGAILT
210 220 230 240 250
PKLNAITLVP MFPHTLSSRP IVLDADSEVR LLVSPDNQDD AMQVSCDGQV
260 270 280 290
TLAVHPGDEI LIKKSKHKLH LVHPLDYSYF HVLRNKLGWG SKLF
Length:294
Mass (Da):32,155
Last modified:May 15, 2007 - v1
Checksum:i4908E4355FCC7DB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90999.1.
RefSeqiWP_005312583.1. NC_009348.1.
YP_001142747.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO90999; ABO90999; ASA_2998.
GeneIDi4995764.
KEGGiasa:ASA_2998.
PATRICi20792417. VBIAerSal2987_2983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90999.1.
RefSeqiWP_005312583.1. NC_009348.1.
YP_001142747.1. NC_009348.1.

3D structure databases

ProteinModelPortaliA4SQ27.
SMRiA4SQ27. Positions 4-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_2998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO90999; ABO90999; ASA_2998.
GeneIDi4995764.
KEGGiasa:ASA_2998.
PATRICi20792417. VBIAerSal2987_2983.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciASAL382245:GJJN-2985-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449.

Entry informationi

Entry nameiNADK_AERS4
AccessioniPrimary (citable) accession number: A4SQ27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: February 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.