Reviewed, UniProtKB/Swiss-Prot A4SPN5 (SYD_AERS4)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	ASA_2844

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000006629

Sequences

	Sequence		Length	Mass (Da)
	A4SPN5-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: B50A008E8D9EE40B	FASTA	588	65,386
	10 20 30 40 50 60 MRSIYCGQVN EAHAGQTITL CGWVHRRRDL GGLIFIDMRD REGIVQVFFD PDKPDAFALA 70 80 90 100 110 120 SELRGEFCIQ VSGVVRTRPD SQRNSDMATG AIEVFAHELT IINRAEPLPL DFNQVNSEEQ 130 140 150 160 170 180 RLKFRYLDLR RPEMAKYLKT RAKASAFVRR FMDEHGFLDI ETPMLTKATP EGARDYLVPS 190 200 210 220 230 240 RVHKGKFYAL PQSPQLFKQL LMMSGFDRYY QIVKCFRDED LRADRQPEFT QIDVETSFMT 250 260 270 280 290 300 APQVRELMEE MIRNLWQHVL AVDLGDFPVM TFDEAMRRFG SDKPDLRLPM ELVDVADLLT 310 320 330 340 350 360 AVEFAVFAGP ANDPKGRVAA LKVPGGAELS RKQIDEYTKF VGIYGAKGLA WMKVNEAANG 370 380 390 400 410 420 IEGVQSPVAK FLSDEIVREI LARTGAADGD IIFFGADSKK VVADAIGALR LKVGRDLGLM 430 440 450 460 470 480 ENSWKPLWVI DFPMFEEDSE GGLAAMHHPF TAPSNLGPSE LKANPLSAYA NAYDMVINGY 490 500 510 520 530 540 EVGGGSVRIH NSEMQATVFD ILGITPAEQR LKFGFLLDAL KYGTPPHAGL AFGLDRLSML 550 560 570 580 LTGTDNIRDV IAFPKTTAAA CLMTDAPSFA NQAQMSELAI ATTVKGDE

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO90857.1.
RefSeq	YP_001142605.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SPN5.
Genome annotation databases
GeneID	4996162.
GenomeReviews	Gene locus ASA_2844 in contig CP000644_GR.
KEGG	asa:ASA_2844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AERS4

Accession

Primary (citable) accession number: A4SPN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)