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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:ASA_2771
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_100006970831 – 829Periplasmic nitrate reductaseAdd BLAST799

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PRIDEiA4SPG7.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi382245.ASA_2771.

Structurei

3D structure databases

ProteinModelPortaliA4SPG7.
SMRiA4SPG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiSTFRHRT.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF199. PTHR11615:SF199. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4SPG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA IKWDKAPCRF
60 70 80 90 100
CGTGCSVLVG SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGEDRLT
110 120 130 140 150
QPMLRMTNGK FDKNGDFAPV SWDQAFDIMA EKFKATLKEK GPTAVGMFGS
160 170 180 190 200
GQWTVWEGYA AAKLMKAGFR TNNLDPNARH CMASAVVGFM RTFGMDEPMG
210 220 230 240 250
CYDDIEQADA FVLWGSNMAE MHPILWSRMS DRRLSNPDVQ VHVLSTFEHR
260 270 280 290 300
SFELADNGMV FTPQTDLAIL NYVANYIIQN DKVNWEFVNK HTQFRKGTTD
310 320 330 340 350
IGYGLRPTHP TEARAKNPGN GDATPMSFDD FAKFVADYDL ESVSKLSGVS
360 370 380 390 400
KEKLEKLAQL YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS
410 420 430 440 450
TPGSGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVTDPKH RAIAEKIWKL
460 470 480 490 500
PEGTIPEQVG YHAVLQNRML KDGKLNAYWV MCNNNMQAGP NMNEEALPGY
510 520 530 540 550
RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA ERRTQFWHQQ
560 570 580 590 600
VKPPEGAKSD LWQLMEFAKR FKVEEVWPAE LIAKQPELKG KTLFDVLYAN
610 620 630 640 650
GQVDQFPKEQ SKGEFNDESE SFGFYVQKGL FEEYATFGRG HGHDLAPFDQ
660 670 680 690 700
YHEARGLRWP VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGRAVIFAL
710 720 730 740 750
PYEPAAEAPD KEYDMWLSTG RVLEHWHTGT MTRRVPELYR AFPDAVLFMH
760 770 780 790 800
PEDAKARGVR RGEEVIVSSR RGEVKTRVET RGRNRPPKGL VFMPFFDASQ
810 820
LVNKLTLDAT DPLSKETDYK KCAVKVVKA
Length:829
Mass (Da):92,835
Last modified:May 15, 2007 - v1
Checksum:i932C11A4DDA4A200
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90789.1.
RefSeqiWP_005309877.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO90789; ABO90789; ASA_2771.
GeneIDi4998327.
KEGGiasa:ASA_2771.
PATRICi20791939. VBIAerSal2987_2745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90789.1.
RefSeqiWP_005309877.1. NC_009348.1.

3D structure databases

ProteinModelPortaliA4SPG7.
SMRiA4SPG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_2771.

Proteomic databases

PRIDEiA4SPG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO90789; ABO90789; ASA_2771.
GeneIDi4998327.
KEGGiasa:ASA_2771.
PATRICi20791939. VBIAerSal2987_2745.

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiSTFRHRT.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF199. PTHR11615:SF199. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNAPA_AERS4
AccessioniPrimary (citable) accession number: A4SPG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: March 15, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.