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A4SPG7 (NAPA_AERS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:ASA_2771
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 829799Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069708

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SPG7 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 932C11A4DDA4A200

FASTA82992,835
        10         20         30         40         50         60 
MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA IKWDKAPCRF CGTGCSVLVG 

        70         80         90        100        110        120 
SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGEDRLT QPMLRMTNGK FDKNGDFAPV 

       130        140        150        160        170        180 
SWDQAFDIMA EKFKATLKEK GPTAVGMFGS GQWTVWEGYA AAKLMKAGFR TNNLDPNARH 

       190        200        210        220        230        240 
CMASAVVGFM RTFGMDEPMG CYDDIEQADA FVLWGSNMAE MHPILWSRMS DRRLSNPDVQ 

       250        260        270        280        290        300 
VHVLSTFEHR SFELADNGMV FTPQTDLAIL NYVANYIIQN DKVNWEFVNK HTQFRKGTTD 

       310        320        330        340        350        360 
IGYGLRPTHP TEARAKNPGN GDATPMSFDD FAKFVADYDL ESVSKLSGVS KEKLEKLAQL 

       370        380        390        400        410        420 
YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS TPGSGPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFAHRLP ADMVVTDPKH RAIAEKIWKL PEGTIPEQVG YHAVLQNRML KDGKLNAYWV 

       490        500        510        520        530        540 
MCNNNMQAGP NMNEEALPGY RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWHQQ VKPPEGAKSD LWQLMEFAKR FKVEEVWPAE LIAKQPELKG KTLFDVLYAN 

       610        620        630        640        650        660 
GQVDQFPKEQ SKGEFNDESE SFGFYVQKGL FEEYATFGRG HGHDLAPFDQ YHEARGLRWP 

       670        680        690        700        710        720 
VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGRAVIFAL PYEPAAEAPD KEYDMWLSTG 

       730        740        750        760        770        780 
RVLEHWHTGT MTRRVPELYR AFPDAVLFMH PEDAKARGVR RGEEVIVSSR RGEVKTRVET 

       790        800        810        820 
RGRNRPPKGL VFMPFFDASQ LVNKLTLDAT DPLSKETDYK KCAVKVVKA 

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References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000644 Genomic DNA. Translation: ABO90789.1.
RefSeqYP_001142537.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4SPG7.
SMRA4SPG7. Positions 40-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382245.ASA_2771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO90789; ABO90789; ASA_2771.
GeneID4998327.
KEGGasa:ASA_2771.
PATRIC20791939. VBIAerSal2987_2745.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycASAL382245:GJJN-2760-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_AERS4
AccessionPrimary (citable) accession number: A4SPG7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families