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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei484Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei533Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei560Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei795Substrate; via amide nitrogenUniRule annotation1
Binding sitei803Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei820Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:ASA_2771
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_100006970831 – 829Periplasmic nitrate reductaseUniRule annotationAdd BLAST799

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiA4SPG7

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi382245.ASA_2771

Structurei

3D structure databases

ProteinModelPortaliA4SPG7
SMRiA4SPG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni510 – 511Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni719 – 728Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4SPG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA IKWDKAPCRF
60 70 80 90 100
CGTGCSVLVG SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGEDRLT
110 120 130 140 150
QPMLRMTNGK FDKNGDFAPV SWDQAFDIMA EKFKATLKEK GPTAVGMFGS
160 170 180 190 200
GQWTVWEGYA AAKLMKAGFR TNNLDPNARH CMASAVVGFM RTFGMDEPMG
210 220 230 240 250
CYDDIEQADA FVLWGSNMAE MHPILWSRMS DRRLSNPDVQ VHVLSTFEHR
260 270 280 290 300
SFELADNGMV FTPQTDLAIL NYVANYIIQN DKVNWEFVNK HTQFRKGTTD
310 320 330 340 350
IGYGLRPTHP TEARAKNPGN GDATPMSFDD FAKFVADYDL ESVSKLSGVS
360 370 380 390 400
KEKLEKLAQL YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS
410 420 430 440 450
TPGSGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVTDPKH RAIAEKIWKL
460 470 480 490 500
PEGTIPEQVG YHAVLQNRML KDGKLNAYWV MCNNNMQAGP NMNEEALPGY
510 520 530 540 550
RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA ERRTQFWHQQ
560 570 580 590 600
VKPPEGAKSD LWQLMEFAKR FKVEEVWPAE LIAKQPELKG KTLFDVLYAN
610 620 630 640 650
GQVDQFPKEQ SKGEFNDESE SFGFYVQKGL FEEYATFGRG HGHDLAPFDQ
660 670 680 690 700
YHEARGLRWP VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGRAVIFAL
710 720 730 740 750
PYEPAAEAPD KEYDMWLSTG RVLEHWHTGT MTRRVPELYR AFPDAVLFMH
760 770 780 790 800
PEDAKARGVR RGEEVIVSSR RGEVKTRVET RGRNRPPKGL VFMPFFDASQ
810 820
LVNKLTLDAT DPLSKETDYK KCAVKVVKA
Length:829
Mass (Da):92,835
Last modified:May 15, 2007 - v1
Checksum:i932C11A4DDA4A200
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA Translation: ABO90789.1
RefSeqiWP_005309877.1, NC_009348.1

Genome annotation databases

EnsemblBacteriaiABO90789; ABO90789; ASA_2771
GeneIDi4998327
KEGGiasa:ASA_2771

Similar proteinsi

Entry informationi

Entry nameiNAPA_AERS4
AccessioniPrimary (citable) accession number: A4SPG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health