ID   FTHS_AERS4              Reviewed;         580 AA.
AC   A4SPE5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   27-MAR-2024, entry version 86.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=ASA_2749;
OS   Aeromonas salmonicida (strain A449).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=382245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A449;
RX   PubMed=18801193; DOI=10.1186/1471-2164-9-427;
RA   Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J.,
RA   Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C.,
RA   Brown L.L.;
RT   "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into
RT   the evolution of a fish pathogen.";
RL   BMC Genomics 9:427-427(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000644; ABO90767.1; -; Genomic_DNA.
DR   RefSeq; WP_005309936.1; NC_009348.1.
DR   AlphaFoldDB; A4SPE5; -.
DR   SMR; A4SPE5; -.
DR   STRING; 29491.GCA_000820065_00063; -.
DR   KEGG; asa:ASA_2749; -.
DR   PATRIC; fig|382245.13.peg.2724; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000225; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..580
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300515"
FT   BINDING         68..75
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   580 AA;  60953 MW;  3E6AFCE1DCF8BBA2 CRC64;
     MLSDIEISRQ SPRLSIQALA SRLGIPAHLL SPHGHYKGKL SLELLKQAAS KPGKKAGKLV
     LVSAITPTPL GEGKTVTTLG LSMGLNHIGQ PSMATIRQPS LGPVFGVKGG AAGGGHAQVV
     PMEEMNLHLT GDFHALTAAH NLAAAALDAR LFHETKLGTE FTARTGLARL DIDPANILWP
     RTLDMNERAL RHLTIGQGAA ADGVERQDRF VITAASELMA ILALASDLKD LRARIGRIQL
     ALDSQGQPIT AEQLEVAGAM TVLLKDALQP TLMQTTEQTP VLVHAGPFAN IAHGNSSVIA
     DRMALQLTDY VVTEAGFGSD MGLEKFFNIK HRQSGITPAC VVLVATVRGL KANSGLLDIR
     PGQPLPASLL GEDLPTLEKG CANLAWHIHN ARRYGLPVVV AVNSFPTDSK AELDLLMREA
     RQAGACGAAI STAFVEGGAG ASELARAVVA ACEMPSQIQL LYPDEMSLCA KLATLVECGY
     GGRGVILSDK AHRQLAALSA AGWDHLPICV AKTPLSISHD PARKGVPTDF EVPIDEVKLC
     AGAGFVYALA GPIMTMPGLG SLPAYRHIDI GEDGEIVGLS
//