A4SPE5 (FTHS_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	ASA_2749

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000300515
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SPE5 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 3E6AFCE1DCF8BBA2	FASTA	580	60,953
	10 20 30 40 50 60 MLSDIEISRQ SPRLSIQALA SRLGIPAHLL SPHGHYKGKL SLELLKQAAS KPGKKAGKLV 70 80 90 100 110 120 LVSAITPTPL GEGKTVTTLG LSMGLNHIGQ PSMATIRQPS LGPVFGVKGG AAGGGHAQVV 130 140 150 160 170 180 PMEEMNLHLT GDFHALTAAH NLAAAALDAR LFHETKLGTE FTARTGLARL DIDPANILWP 190 200 210 220 230 240 RTLDMNERAL RHLTIGQGAA ADGVERQDRF VITAASELMA ILALASDLKD LRARIGRIQL 250 260 270 280 290 300 ALDSQGQPIT AEQLEVAGAM TVLLKDALQP TLMQTTEQTP VLVHAGPFAN IAHGNSSVIA 310 320 330 340 350 360 DRMALQLTDY VVTEAGFGSD MGLEKFFNIK HRQSGITPAC VVLVATVRGL KANSGLLDIR 370 380 390 400 410 420 PGQPLPASLL GEDLPTLEKG CANLAWHIHN ARRYGLPVVV AVNSFPTDSK AELDLLMREA 430 440 450 460 470 480 RQAGACGAAI STAFVEGGAG ASELARAVVA ACEMPSQIQL LYPDEMSLCA KLATLVECGY 490 500 510 520 530 540 GGRGVILSDK AHRQLAALSA AGWDHLPICV AKTPLSISHD PARKGVPTDF EVPIDEVKLC 550 560 570 580 AGAGFVYALA GPIMTMPGLG SLPAYRHIDI GEDGEIVGLS

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO90767.1.
RefSeq	YP_001142515.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SPE5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SPE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4995845.
GenomeReviews	Gene locus ASA_2749 in contig CP000644_GR.
KEGG	asa:ASA_2749.
PATRIC	20791897. VBIAerSal2987_2724.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HBG677721.
OMA	NHLHHGN.
PhylomeDB	A4SPE5.
ProtClustDB	PRK13506.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_2749-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_AERS4

Accession

Primary (citable) accession number: A4SPE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program