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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciASAL382245:GJJN-2738-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:ASA_2749
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000225: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Formate--tetrahydrofolate ligasePRO_0000300515Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_2749.

Structurei

3D structure databases

ProteinModelPortaliA4SPE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiGNSKSGE.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SPE5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSDIEISRQ SPRLSIQALA SRLGIPAHLL SPHGHYKGKL SLELLKQAAS
60 70 80 90 100
KPGKKAGKLV LVSAITPTPL GEGKTVTTLG LSMGLNHIGQ PSMATIRQPS
110 120 130 140 150
LGPVFGVKGG AAGGGHAQVV PMEEMNLHLT GDFHALTAAH NLAAAALDAR
160 170 180 190 200
LFHETKLGTE FTARTGLARL DIDPANILWP RTLDMNERAL RHLTIGQGAA
210 220 230 240 250
ADGVERQDRF VITAASELMA ILALASDLKD LRARIGRIQL ALDSQGQPIT
260 270 280 290 300
AEQLEVAGAM TVLLKDALQP TLMQTTEQTP VLVHAGPFAN IAHGNSSVIA
310 320 330 340 350
DRMALQLTDY VVTEAGFGSD MGLEKFFNIK HRQSGITPAC VVLVATVRGL
360 370 380 390 400
KANSGLLDIR PGQPLPASLL GEDLPTLEKG CANLAWHIHN ARRYGLPVVV
410 420 430 440 450
AVNSFPTDSK AELDLLMREA RQAGACGAAI STAFVEGGAG ASELARAVVA
460 470 480 490 500
ACEMPSQIQL LYPDEMSLCA KLATLVECGY GGRGVILSDK AHRQLAALSA
510 520 530 540 550
AGWDHLPICV AKTPLSISHD PARKGVPTDF EVPIDEVKLC AGAGFVYALA
560 570 580
GPIMTMPGLG SLPAYRHIDI GEDGEIVGLS
Length:580
Mass (Da):60,953
Last modified:May 15, 2007 - v1
Checksum:i3E6AFCE1DCF8BBA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90767.1.
RefSeqiWP_005309936.1. NC_009348.1.
YP_001142515.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO90767; ABO90767; ASA_2749.
GeneIDi4995845.
KEGGiasa:ASA_2749.
PATRICi20791897. VBIAerSal2987_2724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO90767.1.
RefSeqiWP_005309936.1. NC_009348.1.
YP_001142515.1. NC_009348.1.

3D structure databases

ProteinModelPortaliA4SPE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_2749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO90767; ABO90767; ASA_2749.
GeneIDi4995845.
KEGGiasa:ASA_2749.
PATRICi20791897. VBIAerSal2987_2724.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiGNSKSGE.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciASAL382245:GJJN-2738-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449.

Entry informationi

Entry nameiFTHS_AERS4
AccessioniPrimary (citable) accession number: A4SPE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 15, 2007
Last modified: February 4, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.