Reviewed, UniProtKB/Swiss-Prot A4SNY1 (ARGA_AERS4)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	ASA_2577

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (argJ), argA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084810
Regions
	Domain	295 – 442	148	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	A4SNY1-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: C103030410D78D4A	FASTA	442	49,346
	10 20 30 40 50 60 MREREPSLVY AFRQSTPYVN VHRGATFVLM MGGEAICHPN FANIVSDIAL LQTLGIRLVL 70 80 90 100 110 120 VFGSRPQNDE ALARAGIEAQ YHRRIRVTDD ESFAIIKQVC GGLQYDITAQ LSMGLANTPM 130 140 150 160 170 180 QEARISVVSG NFVTAQPLGV DDGIDFCHSG RVRRIDVEGI TRQLDQKGLV LISPIGCSVT 190 200 210 220 230 240 GESFNLSSEE VARRVAVDLK ADKLICFSST QGVMDRHGEA ISELFPEQAE ELLVELEQAG 250 260 270 280 290 300 EEMSGTARYL RAAIASCRGG VPRSHLVSYQ DDGAMLQELF SRDGLGTQIV RESAEQARAA 310 320 330 340 350 360 TIEDIGGILD LIRPLEEDGI LVRRSREQLE MEIDKFTIIE RDGLIIGCAA LYCFMEEAMA 370 380 390 400 410 420 EMACVAIHPE YRNSNRGDQL VAKVAERAKR LGIRRLFVLT TRSIHWFRER GFDPLEVEDL 430 440 PVERQRLYNW QRRSKVLSKT IS

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO90603.1.
RefSeq	YP_001142351.1.
3D structure databases
SMR	A4SNY1. Positions 2-293, 7-442.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SNY1.
Genome annotation databases
GeneID	4996077.
GenomeReviews	Gene locus ASA_2577 in contig CP000644_GR.
KEGG	asa:ASA_2577.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG545264.
OMA	APYIHAF.
PhylomeDB	A4SNY1.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_AERS4

Accession

Primary (citable) accession number: A4SNY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)