Reviewed, UniProtKB/Swiss-Prot A4SNB5 (METE_AERS4)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	ASA_2338

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017216
Sites
	Metal binding	640	1	Zinc By similarity
	Metal binding	642	1	Zinc By similarity
	Metal binding	725	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SNB5-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 697563AF2222DDC3	FASTA	754	84,633
	10 20 30 40 50 60 MTRAHTLGFP RIGAKRELKF ALESYWRGET TQAELIATGK SLRERHWQAQ RTAGVNLLPV 70 80 90 100 110 120 GDFAWYDQVL GTSLLVDAVP ARHRHGDTDL DTLFRVARGR APTGPSAAAA EMTKWFNTNY 130 140 150 160 170 180 HYLVPEFSRD QRFKLGWSQL FDEVEEAKAL GLPVKAVLLG PVSYLWLGKE KESGFSRLEL 190 200 210 220 230 240 LDRLLIVYQE ILAKLAAQGV EWVQIDEPAL ALDLPDEWRL AYLNAYERLS GPCKLLLTTY 250 260 270 280 290 300 FGSVAHQRDI ITSLKVDGLH LDLVAAPEQL ETLVPHLPAQ WVLSAGVING RNVWRANLAK 310 320 330 340 350 360 LAPTLKALKE KLGERLWVAS SCSLLHSPVD LTLEHELDPQ TRSWFAFALQ KCYELGLLSD 370 380 390 400 410 420 YLDGGDLDKI TAYSQPLVDR ESDSRVHKKA VQSRLASLTN SDFDRQSAYP VRAQAQRNDL 430 440 450 460 470 480 KLPLLPTTTI GSFPQTSEIR VLRQEWRAGR IDDSAYEAGI QAQIKDAIER QEAIGLDVLV 490 500 510 520 530 540 HGEAERNDMV EYFGELLDGF AITRFGWVQS YGSRCVKPPV ITTDIDRPTP MTLEWTKFAQ 550 560 570 580 590 600 SLTDKPVKGM LTGPVTILCW SFPREDVSRE QSALQIGLAI RDEVADLEAA GIKIIQIDEP 610 620 630 640 650 660 AIREGLPLRK QDHQAYLDWA VRAFRLSASP VVDSTQIHTH MCYSDFNLII EAVAALDADV 670 680 690 700 710 720 ITIETSRSQM QLLEAFERFN YPNEIGPGVY DIHSPNVPSQ GWIEDLIRKA AKQIPADRLW 730 740 750 VNPDCGLKTR GWEETEAALK VMVNATKALR TELA

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO90387.1.
RefSeq	YP_001142135.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SNB5.
Genome annotation databases
GeneID	4995094.
GenomeReviews	Gene locus ASA_2338 in contig CP000644_GR.
KEGG	asa:ASA_2338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_AERS4

Accession

Primary (citable) accession number: A4SNB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)