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A4SN40 (DDL_AERS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:ASA_2261
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074761

Regions

Domain120 – 326207ATP-grasp
Nucleotide binding150 – 20556ATP By similarity

Sites

Metal binding2801Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 2 By similarity
Metal binding2951Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SN40 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: FDFAB584D6F8DF10

FASTA32936,361
        10         20         30         40         50         60 
MKNMHVLLLC GGGGSEHEVS LRSANFLEQQ LGLVPGVEVT RVEMFADRWF SADGRECKLG 

        70         80         90        100        110        120 
LDKLLSFDSV ARPVDYVVPC IHGYPGETGD LQSFLELAGL PYLGCDAEAS KICFNKISTK 

       130        140        150        160        170        180 
LWLSAIGIPN TPYLFLTEQN DAALTEAKAA LAKWGKVFIK AASQGSSVGC YSASNETDLL 

       190        200        210        220        230        240 
QGIKDAFGYS EQVLIEKAVK PRELEVAVYQ YGDELIATYP GEICVPQDKF YTYEEKYSSA 

       250        260        270        280        290        300 
SHTATSLKAE GLTQAQADAI HEYALKAFRQ LKLTHLSRID FFLTEEGEIL LNEINTFPGM 

       310        320 
TSISMFPKLL EHHGHSFAHY LEQILRKPA 

« Hide

References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000644 Genomic DNA. Translation: ABO90312.1.
RefSeqYP_001142060.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4SN40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382245.ASA_2261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO90312; ABO90312; ASA_2261.
GeneID4996650.
KEGGasa:ASA_2261.
PATRIC20790860. VBIAerSal2987_2215.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycASAL382245:GJJN-2250-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_AERS4
AccessionPrimary (citable) accession number: A4SN40
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways